Product Catalog

Table of Contents About Solis BioDyne 1 Our Services 4 Unique & Patented Stability TAG Technology New Products 7 Endpoint PCR Enzymes and Master Mixes: Product Selection Guide Hot-start PCR Mixes Standard PCR Mixes PCR Enzymes qPCR Mixes: Product Selection Guides 18 Dye-based qPCR: Mix Compatibility Table 19 Dye-based qPCR Mixes Probe-based qPCR: Mix Compatibility Table 25 Probe-based qPCR Mixes First-strand cDNA Synthesis 31 FIREScript® Reverse Transcriptase and cDNA Synthesis Kits and Mixes RiboGrip™ RNase Inhibitor 1-step RT-qPCR Kits: Product Selection Guide Dye-based RT-qPCR Kit Probe-based...

About Solis BioDyne Established in 1995 Supplier of room-temperature stable PCR reagents Trusted trademark in 110+ countries ISO 9001 and ISO 13485 certified Commitment to quality Quality has always been the core value of our work. To ensure we meet your quality requirements in Research and Diagnostics, we implemented and follow ISO standards. • • • • Proven lot-to-lot consistency and high quality Total control over manufacturing process Supply chain security and traceability Manufacturing process consistency In silico protein design Protein production and purification Assay and product design...

About Solis BioDyne Product Portfolio Reference: Excellent product quality along with affordable prices and committed customer service: these are the reasons why Solis BioDyne is our strategic enzyme supplier since many years. Now we continue our partnership with the robust, stable and very sensitive SolisGreen mixes for our qPCR product platform. DAVIDE ROASCHIO Scientist in Product Development Loewe Biochemica GmbH, Germany Sharing 30 years of expertise for your success In every step of the process you are supported and consulted by a Technical Support Team. From choosing the right product...

PRODUCT CATALOGUE About Solis BioDyne Solis BioDyne as your business partner Reference: Strategic cooperation with the Solis BioDyne team has been one of the most efficient we have experienced during our COVID-19 diagnostic kits products development. Their room temperature stable mastermix is an excellent product which has helped us to reach higher sensitivity and stability of our qPCR diagnostic kits. PETER KILIAN Chief Operating Officer at MultiplexDX Collaboration partners: • Leading kit manufactures in Europe with private & government contracts • Local SME's to branches of global corporations...

About Solis BioDyne Our services Solis BioDyne has always welcomed all innovative ideas with open hands. In order to elevate our mission and introduce new solutions to the field of genetic testing, we are delighted to present to you our OEM service offerings. Learn how you can implement the Stability TAG technology into your business. Contract manufacturing Product development • You are looking for an experienced manufacturer of enzymes and proteins • You are looking for a primary/secondary production site for your protein • You are looking for competence in codon optimization for production...

PRODUCT CATALOGUE Proteins designed to withstand higher temperatures 3D MODEL OF FIREPOL® DNA POLYMERASE INCLUDING STABILITY TAG Thermus aquaticus DNA polymerase Unique & Patented Stability TAG Technology Increased stability and overall shelf-life Compatibility with high throughput robotic systems Increased freeze-thaw cycle tolerance Environmentally friendly cold chain free shipping Safe storage Convenient reaction set-up Our reagents remain fully active even after a power outage has damaged everything else in your freezer, after someone has forgotten the reagents on the table overnight, or...

STABILITY OF HOT FIREPOL® DNA POLYMERASE Stability TAG ensures product stability at room temperature* for 30 days HOT FIREPol® Probe Universal qPCR Mix shows no loss of activity after incubation at room temperature for 1 month (upper graph) compared to storing at –20⁰C (lower graph). All enzymes produced at Solis BioDyne, including DNA polymerases and reverse transcriptases, as well as other proteins (i.e. RNase inhibitor, Uracil-N-glucosylase), are exceptionally stable at room temperature due to a proprietary genetic modification in the polypeptide structure - Stability TAG (EU Patent EP2501716,...

PRODUCT CATALOGUE New products SolisFAST® Lyo-Ready qPCR Kit with UNG • Optimized solution for fast and sensitive amplification and quantitation of DNA targets with probe-based assays • Enables sensitive and specific DNA detection • Contains dUTPs and UNG to prevent carryover contamination • Compatible with fast cycling – results in 30 minutes • Performance preserved after lyophilization • Compatible with lyophilization to beads or cakes Read more page 40 RiboGrip® RNase Inhibitor (220U/µl) • • • • • Novel in silico engineered, protein-based RNase inhibitor Exceptional stability – stable for...

Endpoint PCR Product Selection Guide Product Selection Guide: Endpoint PCR Enzymes and Master Mixes Hot Start SolisFAST® Master Mix Ready To Load SolisFAST® Master Mix with UNG SolisFAST® Master Mix with UNG Ready To Load HOT FIREPol® Multiplex Mix Ready To Load HOT FIREPol® Blend Master Mix HOT FIREPol® Blend Master Mix Ready To Load Amplification Rangea Resulting ends GC-rich performance FIREPol® Master Mix FIREPol® Master Mix Ready To Load Enables amplification of up to 5 kb fragments from low complexity DNA templates (e.g. cDNA, lambda, plasmid DNA), and up to 3

PRODUCT CATALOGUE SolisFAST PCR Master Mixes SolisFAST® Master Mixes are suitable for ‘slow’ and ‘fast’ PCR cyclers. HIRA gene fragment (515 bp) from human gDNA was amplified using SolisFAST® Master Mix (lane 1-2) and Master Mix with UNG (lane 3-4). Fast cycling settings (initial activation 1 min at 98°C; denaturation 5 sec at 98°C, annealing/extension 20 sec at 60°C, 25 cycles) were used on Biometra T1 Thermocycler (lane 1 and 3) and Eppendorf® Mastercycler® X50s (lane 2 and 4). Description A ready-to-use 5x-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR...