SOLIS BIODYNE OÜ A: Teaduspargi 9, 50411 Tartu, Estonia E: [email protected] T: +372 7409 960 F: +372 7402 079 SOLIScript 1-step Probe Kit & Multiplex Probe Kit ® Sensitive and Reliable RNA quantification solisbiodyne.com SOLIScript® 1-step Kits are designed for highly specific cDNA synthesis and probe-based qPCR in a single tube. • SOLIScript® 1-step Probe Kit is best for quantifying 1 to 2 RNA targets. • SOLIScript® 1-step Multiplex Probe Kit is specifically designed for multiplex assays and amplifies equally well up to 4 RNA targets in a single reaction. • • • • Did you know? All Solis BioDyne reagents are shipped without ice reducing shipping cost up to 4x cDNA synthesis up to 60°C for superior specificity Analyze 1-4 targets in 1 reaction Unique 30 days stability at room temperature Shipping without ice Choose one-step format for speed and reliability 3. qPCR reaction set-up

SOLIScript® 1-step Multiplex Probe Kit Analyze up to 2 targets in 1 reaction High specificity and sensitivity Contains ROX passive reference dye One kit for all cyclers Did you know? The possibility of room temperature reaction set-up makes Solis BioDyne products perfect for high throughput applications Analyze 1-4 targets in 1 reaction Robust with GC-rich targets Contains dUTP for UNG treatment Optional ROX or Purple passive reference dye Figure 1. SOLIScript® 1-step Probe Kit was used to perform 2-plex one-step RT-qPCR with five tenfold serial dilutions of human total RNA. Reactions were performed...