SOLIS BIODYNE OÜ A: Teaduspargi 9, 50411 Tartu, Estonia E: [email protected] T: +372 7409 960 F: +372 7402 079 Highly sensitive dye-based one-step RT-qPCR SOLIScript® 1-step SolisGreen® Kit is an optimized solution for highly sensitive real-time one-step RT-PCR (RT-qPCR) using intercalating dye detection via the SYBR®/FAM fluorescence channel. Contains all the components necessary, except RNA template and primers, to perform cDNA synthesis and qPCR in a single tube. Accurate quantification across a broad dynamic range Gene expression analysis and absolute quantification Pathogen detection and quantification Low-copy gene detection Gene knockdown validation miRNA profiling and quantification GMO characterization 30-day stability at room temperature! Figure 1. One-step RT-qPCR targeting PPIA (Peptidylprolyl Isomerase A) gene fragment (101 bp) using eight 10-fold serial dilutions (0.1 pg–1 μg, six replicates at each concentration) of Human Reference RNA (total RNA pooled from 10 human cell lines). Reactions were run on Applied BioSystems™ QuantStudio™ 6 Flex qPCR cycler using the following protocol: 15 min RT at 50 °C, 10 min enzyme activation at 95 °C, followed by 40 cycles of 95 °C for 10 sec, 60 °C for

Sensitive amplification and bright fluorescent signal Figure 2. The human B2M (Beta-2-Microglobulin) target was quantified using five 10-fold serial dilutions (10 pg–100 ng, three replicates at each concentration) of Human Reference RNA with either the SOLIScript® 1-step SolisGreen® Kit (blue) or 1-step dye-based RT-qPCR kit from Competitor BR (red). Reactions were run on Applied Biosystems™ QuantStudio™ 6 Flex according to each manufacturer’s protocol. Kit components: • 40x One-step SOLIScript® Mix contains an in silicoengineered SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor....