SoliSD™ Lyo-compatible Bsm DNA polymerase Kit Catalogue Number Shipping: At room temperature Batch Number and Expiry Date: Store at –20°C upon receipt Routine storage at –20°C (–28°C to –18°C) until Expiry Date Stable at 4°C (2°C to 8°C) for up to 6 months Stable at room temperature (15–25°C) for at least 1 month Freeze-thaw stability: 10 cycles Reaction setup: At room temperature Manufactured by Solis BioDyne, in compliance with the ISO 9001 and ISO 13485 certified Quality Management

Product description: • SoliSD™ Bsm DNA polymerase is based on the Large Fragment of the DNA polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. It has enhanced stability due to the incorporated Stability TAG (covered by the patent EP2501716)**. • The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. It is designed for applications requiring synthesis through double-stranded DNA regions, such as loopmediated isothermal amplification (LAMP). • Unique SoliSD™ Supplement system:...

Features 1. Enzyme is active at a wide temperature range between 5162°C with optimum at 60°C 2. No 5’→3’ and 3’→5’ exonuclease activity 3. Strong strand displacement DNA polymerase activity 4. Short 4-20 minute reaction time to result 5. No NTC signal 6. Increased thermostability for at least 1 month at 37°C’ 7. Market-level inhibitor tolerance Reagents supplied: Component SoliSD™ Bsm DNA polymerase (40 U/µl) 10x Isothermal Reaction Buffer (pH 8.8); 200 mM Tris-HCl, 150 mM (NH4)2SO4 ,1% Tween-20, 250 mM KCl

Step-by-step guidelines: 1. Thaw the reagents at room temperature. Mix by vortexing or pipetting up and down, then centrifuge briefly. 2. Prepare a reaction master mix. Add components except for template DNA in the suggested order. Mix by vortexing or pipetting up and down, then centrifuge briefly. Reaction component Nuclease-free water1 Final conc. 10x Isothermal Reaction Buffer 25x SoliSD™ Supplement SoliSD™ Bsm DNA polymerase (40 U/µl) Not included in the kit; 2 Addition of GC-rich Enhancer is optional, consider adding 1x, 2x, or 5x final concentration to the reaction mix in case of a GC-rich...

3. Dispense the reaction mixture and add DNA template. 4. Perform the reaction at 60°C for 10-45 min. SoliSD™ Bsm DNA polymerase is active at 51-62°C. The optimal temperature of the assay may vary depending on the primers and final buffer conditions. 5. If need be, inactivate the enzyme by heating it at 80°C for 10 min. Primer design and validation LAMP assay requires a set of 4-6 primers. We recommend screening several sets of primers to achieve the best sensitivity and eliminate the possibility of the signal in an NTC (no-template control) reaction. To facilitate primer design use dedicated...

Troubleshooting guide No template or non-target template signal • Clean all surfaces with DNase-containing solution. • Substitute reagents and consumables. • Add primers last while preparing the master mix. Do not incubate mix with primers for a prolonged time. • Reduce reaction time. • Inactivate enzyme if the result read out is performed postreaction (e.g. LF, pH-sensitive methods). Time-to-result is not optimal • Use nucleotides diluted in buffer without TE. • Revise sample preparation method. Consider heat treatment and the addition of GC-rich Enhancer. • If EDTA is added to the reaction...

Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SoliSD™ Bsm DNA polymerase gene. Unit definition: The unit is determined in DNA strand displacement assay, where 1 unit of the enzyme catalyzes displacement of 40 nmol of oligonucleotide probe from the complementary DNA strand in 30 min at 60 °C. Safety precautions: Please refer to Safety Data Sheet for more information. Technical support: Contact your sales representative for any questions or send an email to [email protected] For research use only. Not for use in diagnostic procedures. DS-32-22...

*Product stability is assessed using routine QC assays and QC criteria set forth in the product specification and are intended to provide guidelines for shipping and storage conditions only. Customer or its designee shall be responsible for conducting all necessary stability testing applicable to their assay and/or QC criteria, and to comply with any applicable regulatory requirements or guidelines. Such stability testing shall include testing to validate the lead times for shipment, the shelf life of, and the product specifications applicable to shipment, storage and handling of the assay assembled...