SOLIS BIODYNE OÜ E: [email protected] T: +372 7409 960 F: +372 7402 079 Fast singleplex and multiplex endpoint PCR assays! solisbiodyne.com Fast – less time from sample to results Accurate – reliable results in single- and multiplex assays Stable – 1-2 months stable at room temperature* Trustworthy – avoid carry-over contamination with UNG mixes and get reliable results in Sanger sequencing applications Use mixes to reduce set-up time even more. Add only primers, nuclease free water and DNA template. SolisFAST® Master Mix is a 5x-concentrated solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase with the Stability TAG [1][2][3], increased tolerance to inhibitors and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up. * SolisFAST® Master Mixes are 2 months stable at 25°C and SolisFAST® Master Mixes with UNG are 1 month stable at 25°C. Results in 20 minutes! Figure 1. SolisFAST® Master Mixes are suitable for ‘slow’ and ‘fast’ PCR cyclers. HIRA gene fragment (515 bp) from human gDNA was amplified using SolisFAST® Master Mix (lane 1-2) and Master Mix with UNG (lane 3-4). Fast cycling settings (initial activation 1 min at 98 °C; denaturation 5 sec at 98 °C, annealing/extension 20 sec at 60 °C, 25 cycles) were used on Biometra T1 Thermocycler (lane 1 and 3) and Eppendorf® Mastercycler® X50s (lane 2 and 4). PCR cycler BiometraT1 = ‘slow’ machine Eppendorf X50s = ‘fast’ machine Cycler’s ramp rate [1] Kahre, O., Artma, K., Kahre, T. (2015). European Patent No. 2501716 B1. [2] Kahre, O., Artma, K., Kahre, T. (2016). US Patent No. 9,321,999 B2. [3] Kahre, O., Artma, K., Kahre, T. (2017). South Korea Patent

Excellent singleplex and multiplex PCR results! Figure 2. Equal performance of SolisFAST® Master Mix in singleplex and multiplex assay. Eighteen fragments of human gDNA (ranging from 122 to 1340 bp) were amplified in singleplex and multiplex PCR. Amplifications were performed as a single run on Eppendorf® Mastercycler® X50s using 3-step cycling program optimized for multiplex assays (initial activation 2 min at 98 °C; denaturation 10 sec at 98 °C, annealing 10 sec at 60 °C, extension 30 sec at 72 °C (30 cycles). dUTP and UNG don’t affect Sanger sequencing results! Figure 3. Sanger sequencing...