SOLIS BIODYNE OÜ E: [email protected] T: +372 7409 960 F: +372 7402 079 SolisFAST Probe qPCR Mix ® Fast and highly sensitive probe-based qPCR Features • Fast – delivers reproducible qPCR results up to 2x faster • Accurate – reliable quantification in up to 5-plex assays • Sensitive – consistent results with low- and high-copy targets • Trustworthy – robust performance and 3 to 6 months stability at room temperature* Use mixes to reduce set-up time even more. Add only primers, probes, nuclease free water and DNA template. SolisFAST® Probe qPCR Mix is a 5x-concentrated solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining our novel in-silico designed SolisFAST® DNA Polymerase with the Stability TAG [1][2][3], increased tolerance to inhibitors and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice-free shipping and reaction set-up. * SolisFAST® Probe qPCR Mixes are stable for 6 months and SolisFAST® Probe qPCR Mixes with UNG are stable for 3 months at 25°C. Up to 2x less time from sample to results! Standard qPCR reaction Fast qPCR reaction Figure 1. Example of thermal cycling time saving. Duration of a 40-cycle qPCR run with standard thermal conditions using regular qPCR mix (initial activation 10-12 min; denaturation 15 sec, annealing/ extension 40-60 sec) and fast thermal conditions using SolisFAST® Probe qPCR Master Mix (initial activation 2-3 min; denaturation 2-5 sec, annealing extension 10-20 sec). Amplifications were performed on human gDNA. [1] Kahre, O., Artma, K., Kahre, T. (2015). European Patent No. 2501716 B1. [2] Kahre, O., Artma, K., Kahre, T. (2016). US Patent No. 9,321,999 B2. [3] Kahre, O., Artma, K., Kahre, T. (2017). South Korea Patent No. 10-1773636 B1.

Accurate and sensitive qPCR in singleplex and multiplex assays! Slope = -3.314 E = 100.3% R2 = 1.000 FAM Figure 2. Amplification of a 101 bp fragment of PPIA gene using six tenfold dilutions of human cDNA (100 ng – 1 pg, three replicates at each concentration). qPCR was performed on a CFX96™ qPCR cycler (Bio-Rad) using SolisFAST® Probe qPCR Mix (no ROX), with detection in FAM channel. Thermal conditions: activation 30 sec at 95 °C, cycling 2 sec at 95 °C, 10 sec at 60 °C. Avoid carry-over contamination with mixes containing UNG! Figure 3. Four-plex qPCR amplification with four tenfold serial...