SOLIS BIODYNE OÜ E: [email protected] T: +372 7409 960 F: +372 7402 079 SolisFAST qPCR range ® Fast and highly sensitive qPCR range with remarkable stability and inhibitor tolerance solisbiodyne.com A) Test Sample of SolisFAST® Probe qPCR Mix: stored for 6 months at +25°C • Fast – delivers results 2-4x faster • Accurate – reproducible quantification of up to 5- plex assays with probe-based mixes • Sensitive – consistent results with low- and high-copy targets • Trustworthy – increased room temperature stability up to 6 months adds security and flexibility SolisFAST® qPCR range offers ready-to-use probe- and dye-based qPCR mixes for fast, highly sensitive and reproducible qPCR assays. Combining our in silico designed inhibitor tolerant SolisFAST® DNA Polymerase and an optimized buffer, the SolisFAST® qPCR Mixes enable robust qPCR performance and accurate target detection in demanding conditions. The product line offers ice-free shipping and reaction set-up. B) Reference Sample: stored at -20°C only Exceptional stability of the SolisFAST® qPCR range enables shipping at ambient temperature Table 1. Stability features of the entire SolisFAST® qPCR range Figure 1. A Test Sample of the SolisFAST® Probe qPCR Mix (no ROX) was incubated at +25°C for 6 months. A Reference sample of this product was stored at -20°C only. 4-plex qPCR reactions (FAM, blue; VIC, green; JUN, orange; Cy5, purple) using both the Test sample (A, upper graph) and the Reference sample (B, lower graph) were performed on the Bio-Rad CFX96 platform, using three 10-fold serial dilutions of human gDNA (from 2 ng/µl to 0.02 ng/µl). No significant changes in Cq values and fluore

SolisFAST® products have been tested to tolerate common PCR inhibitors, allowing robust performance from complex samples where PCR inhibition is an issue. Table 2. To assess the inhibitor tolerance of the SolisFAST® probe-based qPCR mixes, a qPCR test system, targeting a 72 bp region of human gDNA, was developed and the impact of common PCR inhibitors to the reaction was evaluated. Three gold standard inhibitor tolerant competitor products were also assessed in the same panel. Tolerance limits, meaning the inhibitor concentrations at which the Ct value does not increase by more than 1, are displayed...