Catalog excerpts
FX500 Exchangeable Fluidics Cell Sorter
Open the catalog to page 1FX500 Cell Sorter Sorting Made Simple™ The FX500 is a microfluidics chip based cell sorter featuring a fully replaceable fluidics system. This lets researchers easily replace the entire fluidics path to control sample to sample carryover. The FX500 features comprehensive fluidics controls, advanced automation and easy to use software. Patented CoreFinder™ technology automates instrument setup and streamlines workflow. The optical design offers up to three collinear excitation lasers - 488 nm, 561 nm and 638 nm and six fluorescence detectors. The six free-form PMTs enable detection of...
Open the catalog to page 2Innovative Fluidics The FX500 features a unique replaceable fluidics system which includes an E-beam irradiated sorting chip and PEEK sample line as well as a gamma irradiated sheath line assembly. These single use consumables can be easily exchanged between experimental runs to control the sample to sample carryover. E-beam irradiated sorting chip, PEEK sample line and gamma irradiated sheath line can be easily exchanged between experiments to control sample to sample carryover.
Open the catalog to page 3Sample line Sheath line assembly The sample is introduced into the E-beam irradiated sorting chip via the PEEK sample line. Exchanging these single use consumables between experiments controls sample to sample carryover. The sheath line assembly is made of gammairradiated sheath line that can be linked to multiple sheath bags and connected to the chip via the chip connector. E-beam irradiated PEEK sample line. Gamma irradiated sheath line assembly. The absence of fluidics pumps and inline valves in this design allows for an easy replacement of the sheath line as needed. Chip connector....
Open the catalog to page 4Innovative Fluidics Microfluidics Sorting Chip The E beam irradiated sorting chip acts as an integrated flow cell-nozzle and can be easily installed and removed. The sample and sheath fluid are injected into the chip micro-fluidic channels via inlet ports. Excitation lasers interrogate the sample within the chip before it passes through and is sorted as droplets. The chip is directly connected to the waste line and can be declogged via software options. Unlike traditional systems that require nozzles to be removed for manual cleaning, the FX500 system uses an automated purge and prime cycle...
Open the catalog to page 5Optional Biosafety Solution The BCC300 class A2 Level II biosafety cabinet is available as an option to provide both product and personnel protection. The cabinet measures 1,180 mm (W) x 991 mm (D) x 2,239 mm (H). Researchers can choose from BSC models which have integrated aerosol management system to ensure redundancy in operator protection. Biosafety Standards Compliance The cabinet was tested by the Baker Company with the FX500 sorter inside the work area. The testing validated that the biosafety cabinet with FX500 inside meets National Sanitation Foundation 49 Standard (NSF49), the...
Open the catalog to page 6Automation from Set-up to Analysis Patented CoreFinder technology along with software and sensors provide automation across the workflow from set up to shut down to ensure consistency, save time and improve the accuracy of results. Chip Loading & Positioning Automated Optical Axis Adjustment System set-up begins by installing the sorting chip on the chip loader. Once the chip is loaded, the sheath, sample and vacuum lines are automatically connected and sealed to their respective ports. The chip is automatically aligned to the lasers and optimized during setup using the Sony patented...
Open the catalog to page 7Automatic c*Abr*]’CHi ii*n two sjtcKflfbflf Easy to use software automates sort set up. Automation for Droplet Calibration, Side Stream Calibration and Drop Delay Calibration The droplets are automatically calibrated by adjusting the frequency and the drop drive to achieve optimal break off point (BOP) for each type of sorting chip. Automated side stream calibration is achieved by calculating the angle and the position of the side streams and making adjustments for tube and plate sorting during setup. This ensures that the sort stream is centered in the collection tube automatically without...
Open the catalog to page 8Software A ribbon interface logically organizes features to make them rapidly accessible. An experiment-centric approach makes the software easy to teach, learn and use. The software is intuitive and supports automated setup to acquisition and analysis. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third party analysis tools. System Startup Upon start up, the system initiation includes diagnostics that ensure all subsystems are properly working. Once verified, system status and green ready message are displayed on the LCD monitor on the front of the...
Open the catalog to page 9A ribbon interface logically organizes features to make them rapidly accessible. An experiment-centric approach makes the software easy to teach, learn and use. The software is intuitive and supports automated setup to acquisition and analysis. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third party analysis tools. Software wizards include step-wise workflows that guide users through start up, multicolor compensation, sorting and system shutdown. Creating Experiments Data Analysis and Display Users can create an experiment by using templates from a...
Open the catalog to page 10High purity sorting of T cells In this data, cells from buffy coat preparation from human blood were used at a final concentration of 20 million/ml. Cells were stained with 10 microliters of human anti-CD45 APC (Cat # 2120060), anti-CD3 PE (Cat # 2102040), anti-CD8 FITC (Cat #2323520) and anti-CD4 PECy7 (Cat #2102560) from Sony Biotechnology Inc. were used for staining the cells for 30 minutes. The stained sample was run on FX500 cell sorter using the 70um sorting chip. At an event rate of 20,000 events/s, CD8+ and CD4+ were sorted with a purity of >99% and efficiency of >75%. Single Cells...
Open the catalog to page 11Filter Guide
Open the catalog to page 12* Typical for sample and collection tubes r* Typical for sample tube only ** Calculated using Spherotech beads (RCP-30-5A) according to Sony's specifications
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