MA900
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MA900 Multi-Application Cell Sorter

MA900 Cell Sorter Sorting Made Simple™ The MA900 from Sony meets the needs of most sorting applications, supporting 12 fluorescence parameters and 4-way sorting. Powerful, modern technologies built into the MA900 system include a patented microfluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability. Precision benchtop system that is easy to use and supports 12 fluorescence parameters and 4-way sorting Up to 14 parameters from 4 lasers The MA900 offers choice and...

With the highest level of automation available on any cell sorter, the MA900 uses sensors, software, and engineering know-how across the workflow from startup to aseptic cleaning, QC, and sort setup. Intelligent automation dramatically simplifies operation and streamlines troubleshooting. Initiation Upon startup, the software initiates connection between all subsystems and runs diagnostics that ensure that everything is working properly. Once the subsystems are verified, the software displays status and a green ready message on the instrument’s LCD monitor. Startup automation ensures...

Automated Chip Loading and Positioning Automated Optical Axis Adjustment Automated Droplet Calibration System setup begins with a one-touch installation to load the microfluidic flow cell chip. A setup software wizard guides you through the process of loading a sorting chip, selecting lasers, and inserting the optical filters required. Actuators ensure precise positioning of the chip inside the chip loader. The microfluidic sorting chip uses patented CoreFinder™ technology to automate and streamline key steps in the workflow including chip alignment and accurate calculation of the laser...

The angle and the position of the side streams are calculated and adjusted during setup for tube and plate sorting. This ensures that the sort stream is centered in the collection tube automatically. A dedicated laser and camera perform real-time analysis of droplet images using AutoSetup beads. Patented technologies calculate drop delay to ensure cells are sorted to desired purity and yield. Automated Quality Control Using QC beads, the rCV and linearity are measured and can be displayed in Levey-Jennings plots. This information helps administrators accurately assess optical performance...

The fluidics cart houses autoclavable sheath, deionized water, and waste tanks as well as tanks containing bleach and ethanol. Weighted sensors on the fluidics cart allow a real-time measurement of the fluid levels, which is displayed in the software. Software guides the operation of default and custom fluidic system cleaning. Default cycles include cleaning with bleach or ethanol. Software guides you through each step of the selected cleaning cycle. Fluid is pumped from three cleaning tanks via a cleaning chip. The software also supports user-defined protocols for setting the time for...

Experiment Settings Experiments can be created by selecting a new template, a recent experiment, or a shared template (public) from the experiment window. If a new template is selected, the software guides you through choosing experiment settings such as sample groups, tubes, and pulse parameters for data acquisition. Once a template is selected or created, compensation data can be acquired easily by using the software based compensation wizard. Alternatively, uncompensated populations can be adjusted using the drag and drop feature in the software. During acquisition, data is displayed on...

Sorting Once events are gated on plots, target cells can be sorted for further analysis. Sort mode, sort gates, and sort devices can be assigned using a simple dialog box. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted popu-lation. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates.

Precision engineering for accurate cell deposition and easy to use index sorting software Sort Deposition System The Sort Deposition System is an optional hardware feature that facilitates highthroughput sorting and precise deposition of cells into 6-, 12-, 24-, 48-, 96-, and 384well plates or PCR plates. Several features on the MA900 enable deposition of single cells at a high efficiency. These include the ability to precisely adjust the position of a plate, assign the center of the drop relative to the target cell position, and utilize custom angled plate holders for multiwell PCR and...

Index Sorting Software Index sorting software records the X and Y coordinates of each event sorted into a multiwell device. This very precise and easy to use software brings powerful capabilities to research, enabling you to track the scatter and fluorescence intensity of individual cells sorted in each well. After the sort, the index file can be exported in CSV format or analyzed on the cell sorter. The Well Select feature lets you choose a well and display event(s) sorted in that well on bivariate plots and histograms. The Gate Select feature lets you display the location of events on a...

High-purity 4-way sorting of a 12-color immunophenotyping panel HLA-DR Alexa Fluor® 700

Engineered for flexibility to fit a wide range of applications Whole blood was lysed with RBC Lysis Buffer and stained with Alexa Fluor® 488 CD3, PerCP-Cy™5.5 CD4, BD Horizon Brilliant™ Violet 570 (BV570) CD8, APC-Cy™7 CD14, PE/Dazzle™ 594 CD16, BV421 CD19, BV605 CD20, BV510 CD27, APC CD45RA, PE-Cy7 CD56, Alexa Fluor® 700 HLA-DR, and PE TCR gamma/delta antibodies. Cells were incubated for 20 minutes on ice, washed 2X with staining buffer, and analyzed on the MA900 equipped with 488-nm, 405-nm, and 638-nm lasers. HLA-DR Alexa Fluor® 700 Scatter was used for gating lymphocytes (A). The CD3+...

Ease-of-use from multicolor to single-cell analysis High-purity sorting of a 12-color immunophenotyping panel using 5 violet fluorochromes A A x1,000 HLA-DR Brilliant Violet 711 Whole blood was lysed with RBC Lysis Buffer and stained with the following antibodies: Alexa Fluor® 488 CD3, PE CD19, PerCP-Cy5.5 CD8, PE/Dazzle 594 CD16, BD Horizon Brilliant Violet 421 (BV421) CCR7, BV510 CD27, BV605 CD20, BV650 CD45RA, BV711 HLA-DR, and BV750 CD4. Cells were incubated for 20 minutes on ice, washed 2X with staining buffer, and analyzed on the MA900. Lymphocytes were gated using the scatter gate...