SH800S Cell Sorter Sorting Made Simple™ The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes and applications using the 70 µm, 100 µm, and 130 µm microfluidics sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use. System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third party...

Automation from Set-up to Analysis The SH800S provides the highest level of automation and ease of use available in a cell sorter. Researchers can accurately set-up, calibrate and monitor sorting with a push of a button. This delivers true ease of operation to save time and improve consistency of results. A novel microfluidics sorting chip uses patented CoreFinder™ technology to automate key steps of instrument setup and operation streamlining workflow. The system is versatile, allowing researchers to sort of a wide range of cell sizes to accommodate application requirements. Chip sizes include...

Automated Chip Loading & Positioning Automated Optical Axis Adjustment Automated Droplet Calibration System set-up begins with a one-touch install to load the microfluidics flow cell chip. Actuators ensure precise positioning of the chip inside the chip loader. Sheath, sample and vacuum lines connect and seal automatically to their respective ports once the chip is loaded. Made of durable plastic the chip is easy to replace when needed. The alignment of the chip to the lasers is optimized automatically during setup using the Sony patented Blu-Ray™ technology for aligning and tracking laser position....

Software Software, sensors and CoreFinder patented technology provide automation across the workflow from set up to shut down to ensure consistency, save time and improve the accuracy of results. The interface is designed with ribbon tabs that logically organize features to make them rapidly accessible. An experiment-centric approach makes the software easy to teach, learn and use. Software wizards include step-wise workflows that guide users through start up, multicolor compensation, sorting and system cleaning. Since the system simplifies even advanced sorting techniques it can be used by researchers...

Experiment Settings Data Analysis and Display Users can create an experiment by selecting a new template, a recent experiment, or a shared template (public) from the experiment window. If a new template is selected the user is guided via dialogs to choose experiment settings such as sample groups, tubes and pulse parameters for data acquisition. Once a template is selected the user can start acquisition or compensation wizard. Data is displayed as dot plots and histograms on worksheets and events can be marked using gates. The software has a number of tools to select, adjust, label and measure...

Biosafety Cabinet The BCC300AMS Class II biosafety cabinet custom designed for SH800S by the Baker Company is available as an option to provide personnel and product protection. The cabinet measures 1,180 mm (W) x 991 mm (D) x 2,239 mm (H). Biosafety Safety Standards Compliance The cabinet was tested by the Baker Company using microbiological assays with the SH800S sorter inside the work area. The testing concluded that the Biosafety Cabinet with the SH800S inside met several international biosafety standards including National Sanitation Foundation Standard 49 (NSF49), and the European Standard...

E-Beam Sterilized Consumables Exchangeable Sample Fluidics Versatile Application Support For applications where cross-contamination between samples or with external biological entities are a factor, optional e-beam sterilized consumables including chip, setup beads and sample lines can be used to streamline workflow. The SH800S sorting chip is an integrated flow cell-nozzle assembly. It contains microfluidic channels for controlling the flow of sample and sheath fluid. The sample is interrogated by the lasers within the chip before it passes through the nozzle for sorting. Easy installation Chip...

Applications Resolution and Sensitivity 653 653 Events Events Events Events Events Events Events Events A. Fluorescence sensitivity MESF values measured using Spherotech 8 peak beads are: FITC 120 and PE 110. B. Fluorescence resolution was measured using propidium iodide (PI) stained chicken erythrocyte nuclei (CEN). Coefficient of variation of <2.5% was observed for the G0/G1 peak. Singlets Singlets Singlets Singlets Events Events PE-H-Compensated PE-H-Compensated Events Events Singlets: Singlets: 68.45% 68.45% Singlets: Singlets: 68.45% 68.45% Immunophenotyping Assays Distinct resolution of...

Fluorescent Protein Analysis D Human cell lines co-expressing GFP, YFP, dsRed and CFP using the fluorescent protein optical filters is shown. Refer to the filter guides for the fluorescent protein filter sets. Sorting and Viability Analysis DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated Live Cells Live Cells Live Cells: 87.69% Live Cells: 87.69% FSC-A FSC-A tdTomato-A-Compensated tdTomato-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated DAPI-A-Compensated Sorting and...

Small Particle Resolution High speed sorting of E.coli using 70um sorting chip- Concentrated sample of E.coli cells was sorted at a threshold event rate of 30,000 events/sec at a sort setting of 50kHz and 40psi. Post sort analysis shows that >95% purity was obtained.

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