SP6800
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SP6800 Spectral Analyzer The Sony SP6800 Spectral Analyzer uses spectral technology to optimize sensitivity and enhance dim signal detection by collecting photons from 420nm to 800nm. Spectral technology also simplifies multicolor panel design, by eliminating bandpass filters and conventional compensation matrices while delivering better data and simplifying visualization for the study of heterogeneous populations. Novel global standardization mode automatically sets the system to a master specification with a single click. This capability eliminates instrument variability from day to day...

The 405nm, 488nm and 638nm excitation lasers are positioned to reduce fluorescent noise. They enable the system to support 16 or more fluorescent parameters. The SP6800 spectral flow analyzer improves sensitivity and simplifies application design, workflow, and analysis over conventional flow cytometers. This is achieved using spectral analysis technology, advanced electronics, and patented optical technologies. These capabilities, unique to Sony Biotechnology systems, allow experienced and novice flow cytometrists to achieve greater flexibility for panel design and more accurate...

Software The SP6800 software is easy to learn and use. It guides researchers from set-up to panel design, acquisition, analysis, and shutdown. User preferences allow users and administrators set up options for overall instrument operation and experiment set up to facilitate use and unattended operation procedures. System Start Up At start up Align Check and Performance QC wizards check instrument calibrations, using beads to ensure the instrument is operating optimally. On screen instructions guide the user through procedures, then display progress and report results. The performance report...

Spectral Library Experiment Creation The Spectral Library lets users create a personal reagent library that simplifies experiment creation and saves time. An Acquisition Wizard assists the users with step by step instructions to acquire and analyze single positive controls for your spectral library. Once acquired the spectral reference for that reagent including the spectral index are available for future use. Information from the spectral library is available to users with a simple click improving accuracy and streamline panel design. Experiments can be created using a template, an...

Spectral Analyzers from Sony include FCS Express, from De Novo Software. FCS Express offers a range of new analysis tools from live gating to batch analysis. Native support for Sony Spectral data files to enable sophisticated data transformations and visualizations such as spectral overlays, tSNE, Spade, and heat maps. Dot plots and spectral plots can be set up with live gating. Live gating allows users to find positive and negative populations quickly and easily for added assurance about target populations. Flexible Analysis Batch and Report Flexible data analysis can include integrated...

Spectral Analysis Technology Spectral analysis technology is the foundation of the SP6800 system. Spectral flow cytometry streamlines workflow and yields better data by keeping all the light collected. In conventional systems, overlapping fluorescence is subtracted using color compensation, so less light is collected. Instead, spectral flow cytometers sum the fluorescence together and then use unmixing to mathematically separate the colors. This powerful capability also simplifies workflow including panel design, and improves visualization of autofluorescence. A unique prism collection...

Uniform measurement of Fluorescent Emissions A correction system adjusts the offset and sensitivity of each channel of the 32 channel PMT to ensure a uniform and accurate measurement of fluorescent emission from 420nm to 800nm. The corrective system brings a standardization to all 32 PMTs ensuring the user is getting the most reliable data with the ease of adjusting only one voltage. This saves time over conventional flow cytometry operation where users must calibrate each individual PMT. Post correction Pre and Post Correction Profile. Each graph illustrates pre and post correction in the...

Fluorochrome Panel Guide Application data dye options and combinations. Select one from each group. • Alexa Fluor® 488; 488nm • Brilliant Blue 515; 488nm • FITC; 488nm TM • Alexa Fluor 532; 488nm • Alexa Fluor 514; 488nm • PE Dazzle 594; 488nm • PE Texas Red®; 488nm TM • Brilliant Violet 421; 405nm • Alexa Fluor 405; 405nm TM • Pacific Blue ; 405nm • Horizon V450; 405nm • Brilliant Violet 510 ; 405nm • AmCyan; 405nm • Horizon V500; 405nm • Krome Orange ; 405nm TM

• Brilliant Violet 605TM; 405nm • Brilliant Violet 650TM; 405nm • Brilliant Violet 711TM; 405nm • Brilliant Violet 785TM; 405nm

Sample Data 2 Lasers, 488nm Laser 16 color panel on Human Peripheral Blood Application data using 2 lasers 488nm Laser 488nm Laser 2 Lasers, 405nm Laser 405nm Laser 2 Lasers, Pacific Blue Monocytes Monocytes Monocytes Lymphocytes Lymphocytes Lymphocytes WLSM WLSM WLSM Lymphocytes Lymphocytes Lymphocytes Cytotoxic T Cells Cytotoxic T Cells Cytotoxic T Cells All events All events All events PLASMA B Cells PLASMA B Cells PLASMA B Cells WLSM WLSM WLSM Naive B Cells Naive B Cells Naive B Cells WLSM WLSM WLSM Cytotoxic T Cells Cytotoxic T Cells Cytotoxic T Cells Naive Cytotoxic T Cells Naive...

16 color stained sample of Human Peripheral Blood Application data using 3 lasers 400 488nm Laser Laser 3 Lasers, 488nm Pacific Blue 3 Lasers, 638nm Laser Wavelength (nm) WLSM Monocytes Helper Effector T Cells 1 Helper Naive T Cells Cytotoxic Effector T Cells Cytotoxic Naive T Cells Examples of the detection of several important cell populations using sixteen fluorochromes excited by three lasers. Unlike conventional flow cytometers that can detect up to five off the blue, in this example we demonstrate that with the SP6800 eight fluorochromes can be excited off the blue with clear sample...