THE MULTIPLEX DIGITAL PCR COMPANY CRYSTAL DIGITAL PCR™

CRYSTAL DIGITAL PCR™ Stilla’s Crystal Digital PCR™ technology for absolute quantification of nucleic acids is based on cutting-edge microfluidic technology that integrates the digital PCR process in a single consumable, reducing hands-on time and interaction, so you can simply insert your sample and walk away. The sample is partitioned using a network of microchannels into a large array of individual droplets, also called droplet crystals. The individual droplets are partitioned into identical sizes — each its own reaction compartment — before PCR. PCR then a mplifies the targets in the droplets....

PREPARE I AMPLIFY I READ I ANALYZE The naica® system harnesses the key principles of digital PCR and is designed to provide a sensitive, fast, and easy-to-use solution. Easy-to-use digital PCR solution On-chip integrated workflow Minimal hands-on time Flexible digital PCR Up to 6-color multiplexing From 12 to 48 samples / run Fast time to results Prepare (10 min), Amplify (2 hrs), Read (20 min*), Analyze (5 min*) * Depending on the number of samples analyzed and the number of channels used Crystal Miner software High multiplexing capacity 3- or 6-color system configuration Wide range of fluorophore...

OVERVIEW PREPARE Load the reaction mix into the wells of the chips. naica® PCR MIX reagents are developed for enhanced performance for Crystal Digital PCR™ on the naica® system Place the chips in the Geode. Launch the Crystal Digital PCR™ program. Thousands of droplets are created from each sample PCR amplification is p erformed immediately after the Droplet Crystal is generated Droplet crystals are imaged using up to 6 fluorescent

Measure the concentrations of targeted nucleic acids with Crystal Miner software. Intuitive tool for visual inspection and analysis of Crystal Digital PCRTM experiments Automatic quality control for experiment performance Automatic identification of positive and negative droplets for all fluorescence channels Analyze data using 1D, 2D, or 3D dot plots Inspect Droplet Crystals and link any dot on the dot plates to its corresponding droplet in the Droplet Crystal Obtain targeted nucleic acids concentrations Access raw data and export all experiment details and results * Depending on the number...

SPECIFICATIONS Crystal Digital PCR™ Consumables Capacity Sapphire Chip Ruby Chip Input volume Number of droplets per sample Dynamic range of detection (95%) Crystal Digital PCR™ Reagents naica® multiplex PCR MIX (TaqMan® compatible) Available in both 5X and 10X concentrations For use with probe-based chemistries naica® PCR MIX (EvaGreen® compatible) Available in both 5X and 10X concentrations For use with intercalating dye chemistries Geode Capacity with Sapphire Chip • Up to 12 samples (3 chips) / run • Up to 36 samples / 8h shift (3 runs) Capacity with Ruby Chip • Up to 48 samples (3 chips)...

Scan time < 20 min per run / 3 Sapphire Chips* < 60 min per run / 3 Ruby Chips* Power Supply Scan time < 45 min per run / 3 Sapphire Chips* < 80 min per run / 3 Ruby Chips* Power Supply *Depending on the number of channels scanned Crystal Miner software License & updates fees File format .ncx (naica crystal experiment) .ncr (naica crystal result) Data visualization Droplet crystal imaging; 1D, 2D, 3D plots File size Sapphire Chip: 12 / 249 MB* Ruby Chip: 5 / 19 MB* Export format Compenstion matrix *Max .ncr file size for 3-color / 6-color experi

CRYSTAL DIGITAL PCR™ A RANGE OF APPLICATIONS Experience the flexibility of the naica® system for a wide range of key nucleic acid detection and quantification applications. Oncology Environmental Testing Cell & Gene Therapy Infectious Diseases Gene Editing & Epigenetics To discover more www.stillatechnologies.com Liquid Biopsy For Research Use Only. Not for use in diagnostic procedures.