Catalog excerpts
Crystal Digital PCR is Stilla’s next-generation technology for absolute quantification of nucleic acids. Using cutting-edge microfluidic innovations, this technology integrates the digital PCR process in a single consumable. The sample is first flowed through a network of microchannels and partitioned into a large 2D array of 30,000 individual droplets, also called a droplet crystal. PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the...
Open the catalog to page 2Crystals of 30,000 droplets are created from each sample PCR amplification is performed immediately after crystal generation 2 Read & Analyze • Transfer the chips to the Naica Prism3 crystal reader Crystals are read using 3 fluorescent channels • Measure the concentrations of targeted nucleic acids with Crystal Miner™software
Open the catalog to page 3Sapphire chip Specifications Capacity Input volume Droplets per sample Droplet volume Dynamic range Precision at 95% Cl Naica Geode Specifications Capacity Thermo block temperature range Block uniformity (at 72 °C) Adjustable ramping Technical information Dimensions (W x D x H) Weight Power supply Pressure input Pressurizing gas Up to 12 samples (3 chips) / run 10°C to 95°C ± 0.5 °C 0.1 to 1.0 °C 35 x 37 x 29 cm (14 x 15 x 11 inch) 15 kg 100 - 240 V//50 -60 Hz 1.3 bar (19 psi) Air Specifications Capacity Scan time Sample illumination Excitation wavelengths Detection wavelengths Compatible...
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APPLICATION NOTE
6 Pages