HYBRIDIZATION CAPTURE
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DATA SHEET swiftbiosci.com SWIFT HYBRIDIZATION CAPTURE KITS Targeted Sequencing Panels for Human Exome, Pan-Cancer and Inherited Diseases Swift Hybridization Workflow Introduction Swift Hybridization Capture Panels offer probes for DNA hybridization capture-based target enrichment. The Swift panels consist of individually synthesized and quality-controlled probes designed to provide the highest level of performance and ready to hybridize to NGS libraries to enrich for targets of interest. The Swift Hyb, Wash and Universal Blocker kit completes the target enrichment workflow. Highlights • Optimized hybridization conditions • Efficient 4-hour hybridization reaction with best in class Hyb, wash and blocker reagents • Streamlined workflow • Easy implementation leveraging Swift’s from library to normalization pipeline • Reduced reagent and sequencing costs • Excellent data output with high coverage uniformity and on-target performance • Pre-capture multiplexing conserves hybridization reagents Swift Exome Figure 1. Libraries are pooled and combined with blocking oligos and human Cot DNA. Next, the library pool is hybridized to Swift probes, captured using streptavidin beads, and washed to remove unbound fragments. Then, the enriched library pool is amplified by ® PCR and is ready for multiplexed Illumina sequencing. Swift Pan-Cancer Swift Inherited Diseases Human Target Region Genes Covered Coding region of 19,396 coding genes 127 oncology-related genes* implicated in 12 tumor types 4,503 genes* based on the Human Gene Mutation Database (HGMD®) *See complete gene lists at swiftbiosci.com and to access panel probe a

Swift Hybridization Capture Kits (Continued) Applications and Sample Types • Detection of germline inherited SNVs and Indels • Low frequency somatic variant detection of SNVs and Indels • Copy number variant detection Compatible with the following DNA library prep kits: • Swift 2STM Turbo, for high quality genomic DNA and FFPE • Accel-NGS 2S Hyb for FFPE, cfDNA, and incorporation of molecular identifiers (MIDs) for ultra-low frequency variant detection • Accel-NGS 1S Plus for heavily nicked or denatured samples Figure 2. An independent study, performed by a large genome center, compared the...

Swift Hybridization Capture Kits (Continued) Figure 3. cfDNA was extracted from blood of four individuals with unique genetic background and Coriell gDNA samples from different genetic backgrounds were obtained. To determine the effect of MIDs on low frequency variant calling, sample spike-ins were performed at 1% or 0.5% frequency into 10 ng cfDNA or 100 ng gDNA. Libraries were prepared with the Swift Accel-NGS 2S Hyb Kit with MIDs, enriched with the Swift Pan-Cancer Panel and sequenced on an Illumina HiSeq to a minimum of 8000x coverage. A consensus sequence was generated for each MID...