SWIFT 2S TURBO DNA LIBRARY KITS
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DATA SHEET swiftbiosci.com SWIFT 2S TURBO DNA LIBRARY KITS The Easiest NGS Workflow for Routine Sequencing Highlights • Simple, fast, and reliable Minimal steps and hands-on time with consistent fragmentation regardless of DNA input amount. • For many genomes Compatible with diverse genome types of low or high complexity. • More applications, one workflow One universal approach for whole genome, exome, and large gene studies. The Swift 2S Turbo DNA Library Kits offer a versatile solution that streamlines NGS sample preparation of double-stranded DNA on Illumina® sequencing platforms. This technology leverages rapid and highly reproducible fragmentation and library construction, enabling manual and fully automatable workflows. Enzymatic Prep dsDNA Fragmentation, Repair, & A-tailing  40–60 minutes Illumina TruSeq DNA Single & Combinatorial Dual Indexes 3’ and 5’ ligation of truncated P7 & P5  20 minutes Indexing PCR  Time varies Double-stranded truncated adapters Terminal primers (included) to increase library yie

Swift 2S Turbo DNA Library Kits are available in two configurations to support variety of sample inputs and applications. Swift 2S Turbo is an "all in one" kit for quick implementation of core applications. The Swift 2S Turbo Flexible workflow supports an expanded menu of applications and is compatible with your choice of adapters & indices. Application Swift 2S Turbo Swift 2S Turbo Flexible Whole Genome Sequencing P P Whole Exome Sequencing P P Variant Detection Germline Germline + Somatic Fastest, Easiest Workflow The Swift 2S Turbo leverages a fast, efficient workflow consisting of two...

High Quality Data Swift 2S Turbo offers reproducible and consistent aligned insert sizes across a broad range DNA input amounts. Insert Size Mean base quality Insert Size Insert Size Fraction of normalized coverage Normalized Coverage Windows at GC% − Base Quality at GC% All Reads Level: All Reads GC Bias Plot Insert Size NGS libraries were constructed from high quality genomic DNA (NA12878) for whole genome sequencing. The fragmentation times required to achieve library mode sizes of ~ 350 bp (plus ~ 125 bp, the length of the adapters) were 8- and 10-minutes for DNA ≥ 25 ng and DNA < 25...

Sample Integrity Mean Bait Coverage % Covered % Covered % Covered > 20X > 50X > 100X % Bases On-Target The Swift 2S Turbo was evaluated with the IDT xGen Pan-Cancer probes and DNA inputs from FFPE of different quality. Libraries were sequenced on a MiSeq run with 100 bp PE reads. The integrity of FFPE inputs were qualified via qPCR of Swift Alu 115 repeats. The sample integrity was assessed based on the Alu 247/115 repeat fragment ratios. The fragmentation times were adjusted (10-15 min) for damaged/degraded FFPE targeting library insert size of ~ 200 bp; however, the sequence metrics...

Ordering Information Product Name Swift 2S Turbo Single Indexing Primer Kit Set A Swift 2S Turbo Combinatorial Dual Indexing Primer Kit Swift 2S Turbo DNA Library Kit Swift 2S Turbo Flexible DNA Library Kit Swift 2S Turbo Set S1-S4 Combinatorial Dual Indexing Primer Kits Swift 2S Turbo Unique Dual Indexing Primer Kit Swift 2S Turbo SureSelect Compatibility Module  Visit www.swiftbiosci.com for easy ordering. Swift Biosciences, Inc. 674 S. Wagner Road, Suite 100 • Ann Arbor, MI 48103 • 734.330.2568 • www.swiftbiosci.com © 2018, Swift Biosciences, Inc. The Swift logo is registered trademark...