SWIFT RAPID RNA LIBRARY KIT
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SWIFT RAPID RNA LIBRARY KIT Accelerate Your RNA-Seq Discovery Introduction The Swift Rapid RNA Library Kit offers the fastest and lowest cost NGS transcriptomics workflow for lllumina® sequencing platforms. This kit leverages patented Adaptase' technology, enabling stranded RNA library construction directly from 1st strand cDNA without the cumbersome requirement for 2nd strand cDNA synthesis. The kit is compatible with many upstream and downstream modules and is easily automatable. Reverse Transcription • 90 minutes Fragmentation, Reverse Transcription, Incorporating Truncated Index 2 (i5) Adapter, Bead Clean-up Highlights Adaptase8 • Save time and reduce costs • 30 minutes • Go from RNA to high-quality libraries in 3.5 hours • Supports many workflows, compatible with a variety Indexing PCR of indexing options and Normalase • 40-70 minuti \ Truncated Index 2 (i5) Adapter l /■" 3'Tailing and Ligation of Truncated Index 1 (i7) Adapter Truncated Index 1 / (i7) Adapter Completion of Adapters with Index Sequences, Increase Yields, Bead Clean-up \ Full-length Adapters / Indexed Library Specifications

Save Time with Direct Conversion of 1st Strand cDNA The Swift Rapid RNA Library Kit leverages patented Adaptase® technology to add adapters directly to 1st strand cDNA in an efficient and expedited workflow that does not require adapter titration. Compared to kits supporting the same input ranges, Swift libraries are more economical for both time and cost. Further accelerate your RNA-Seq workflows through automation with our instrument-specific resources and recommendations. Swift Rapid RNA - the fastest RNA library kit, completed in 3.5 hours KEY F = Fragmentation RT= Reverse Transcription...

Consistent Expression Profiling Across Inputs The Swift Rapid RNA Library Kit provides consistency in transcript detection and expression across supported input ranges. Accelerate your research with a rapid workflow that provides reliable and consistent data. FPKM for 500 ng, 250 ng, and 100 ng of Universal Human Reference RNA Inputs of 500 ng, 250 ng, or 100 ng Universal Human Reference Total RNA (Agilent 740000) were poly(A) enriched using the NEBNext poly(A) mRNA Magnetic Isolation Module (NEB E7490) before being processed through the Swift Rapid RNA Library Kit. Libraries were sequenced...

Product Name Kit Size (rxns) Cat. No. Swift Rapid RNA Library Kit 96 R2096 An Indexing Primer Kit is required for complete functionality. Please select from the following: Single Indexing Primer Kit Set A (12 indices, 2 rxns ea) 24 Combinatorial Dual Indexing Primer Kit (96 combinations, 1 rxn ea) 96 24x8 (192) Swift Set S1-S4 Combinatorial Dual Indexing Primer Kits Swift Unique Dual Indexing Primer Kits (96 UDIs) Swift Normalase is compatible with this kit. Please order both of the following: Swift Normalase Kit 96 66096 Normalase Combinatorial Dual Indexing Primer Kit (96 combinations) 96...