CHROMATOGRAPHY SYSTEMS MADE IN GERMANY PREPARATIVE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY SOLUTIONS

HIGH PERFORMANCE SEPARATIONS REVERSED PHASE NORMAL PHASE LIMITLESS CUSTOMIZATION

'watm CHROMATOGRAPHY The Sykam S 700 series of preparative LC modules and preparative LC systems offers the user a wide range of possible system configurations. Whether you need a simple isocratic chromatography system with manual sample injection or a fully automated quaternary high pressure gradient system for multi-dimensional chromatography with column switching and reversed flow capabilities, the S 700 series offers the user maximum flexibility to configure the system for virtually any preparative chromatographic application. Following general features apply to the Sykam S 700 chromatography...

S 1525 & S 1535 PUMP SERIES We believe it is correct to say that the pumps of a preparative chromatography system - if not with any chromatography system - are the heart of the system. Not withstanding that any part of a chromatography system can play an essential role, it is quite undisputed that the pumps are a key component defining any achievable chromatographic performance. In order to achieve maximum performance for each desired flow rate range we propose two pump units, each of which configurable for specific needs. For a flow rate range of up to 150 ml/min or up to 250 ml/min and the...

The S 1535 Preparative LC Pump is available as a 150 ml/min and a 250 ml/min version and a pressure range of 400 bar with stainless steel pump heads, and up to 350 bar with PEEK pump heads. An optional built-in low pressure quaternary gradient mixer gives the user flexibility for running applications needing complex water/solvent gradients as often encountered in straight phase and reversed phase chromatography, as well as reliable gradient formation and robust operation for high salt containing buffer gradients - as often used in protein chromatography applications. Preparative Pump System S...

The S 1525 Preparative LC pump is the largest heavy duty pump of the preparative LC product range. The S 1525 pump is available with four flow rate ranges. These are 150, 250, 500 or 1000 ml per minute. Each available as stainless steel version or as metal-free PEEK versions. The S 1525 LC pump aims to serve the highest demands in terms of operability and longevity at pressures of up to 400 bar, even at the highest flow rates. The oversized motorization and mechanics implemented at the S 1525 pump warrant low maintenance and high durability for demanding chromatographic applications. Max. flow...

Catalog No. Description Specifications S007021 Preparative Pump S 1525-150 150 ml PEEK pump head 250 ml PEEK pump head 500 ml PEEK pump head 1000 ml PEEK pump head Max. flow rate: 150 ml/min Max. pressure: 350 bar (5000 psi) Max. flow rate: 250 ml/min Max. pressure: 350 bar (5000 psi) Max. flow rate: 500 ml/min Max. pressure: 350 bar (5000 psi) Max. flow rate: 1000 ml/min Max. pressure: 350 bar (5000 psi) Update Options/Accessories Catalog No. Description Notes S006988 Quaternary Gradient Mixer S007085 Binary Gradient Mixer S007081 Tertiary Gradient Mixer Low pressure quaternary gradient mixer...

AUTOSAMPLING The choice for a certain strategy of sample injection is largely dependent on the type of application. Such as: • • • High value peptide purifications with repetitive smaller volume injections Large volume injections where one or more sample cartridges need to be switched into the flow path for flash chromatography purifications Biochromatography with protein purification strategies including affinity, ion-exchange, hydrophobic interaction, and gel permeation chromatography Each of the above examples will typically require a different level of flexibility and lead to a specific choice...

The S 5400 Sampling Module with built-in sampling pump and two separate injection valves offers manual syringe injection and variable volume injection controlled by the sampling pump. Sample Pump The S 5400 in combination with the S 5405 Sample Loading rack with 10 closed 50 ml tubes and a 10-port switching valve is a fully automatic large volume sample injection system. HPLC Pump Shown above: the S 5400 sampling flow path. Valve 1 is the manual injection valve. Valve 2 is the automatic injection valve. Valve 1, valve 2 and the sample pump are integral parts of the S 5400 sampling module. Valve...

Sampling Module S 5400 Stainless Steel / PEEK Catalog No. Sampling Module S 5400 SS Sampling Module S 5400 PEEK Stainless steel, 10 ml & 2 ml sample loops PEEK, 10 ml & 2 ml sample loops Technical Specifications Injection accuracy Injection precision Wetted materials Max. pressure PVC, PTFE, stainless steel/PEEK, PPS 250 bar (PEEK / stainless steel) Sampling Rack S 5405 Catalog No.

UV/VIS DETECTORS UV/Vis detection is probably the most applied and easiest to use chromatographic detection method for preparative chromatography. As common as a UV/Vis detector may be for HPLC applications, it is relevant to know that a UV/Vis detector for preparative HPLC must serve some specific demands, different from a general analytical UV/Vis detector. Among these demands are: • • • • An external flow cell for easy access and positioning in any preparative HPLC configuration Allowing high flow rates while maintaining low pressure drop A high pressure rating flow cell to handle possible...

Variable Wavelength UV/Vis Detector S 3255 THE S 3255 UV/VIS DETECTOR The Sykam S 3255 UV/Vis Detector is a single or optional dual channel variable wavelength detector with a wavelength range of 190 to 800 nm. Built around a high quality “moving monochromator” optics for minimal loss of light energy at any chosen wavelength, the result is a UV/Vis detector with an excellent dynamic measuring range and stability for routine applications as well as demanding preparative HPLC applications. Technical Specifications An integrated wavelength program allows for free programming of different wavelengths...