CyFlow® Cube 8 * Configurations with up 8 optical parameters (up to 6 colours) * Choice of 488, 638, 407, 355, 375, 532, 561, 594, 785 nm lasers * Particle size: 0.1 – 100 µm * Fluorescence sensitivity: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE) High performance flow cytometry The CyFlow® Cube 8 is a compact flow cytometer for analysis of single cells and microscopic particles with a high grade of integration. The CyFlow® Cube 8 gives the unique combination of a truly stand-alone system on a small footprint with a modular configuration system with up to 6 colours. The easy-to-use CyView™ software provides instrument control, data acquisition and data storage. The possibility of customized instrument settings facilitates switching between different applications. Furthermore CyFlow® Cube 8 offers the True Volumetric Absolute Counting (TVAC) feature which allows displaying of particle concentrations for any subsets of cells without the need of reference beads, even if defined by a gate at a later time after the acquisition. Optionen * Optional CyFlow® Robby 8 Autoloading Station for well plates and tubes * Optional CyFlow® Sorter for closed, non-destructive cell and particle sorting with high purity Specification Light Sources: * Up to 4 light sources (3 laser + high power UV-LED) * Blue laser: 50mW @488nm, 200mW adjustable @488nm * Red laser: 25mW @638nm, 40mW @640nm * Violet laser: 100mW @405nm * UV laser: 60mW @375nm * Green laser: 30mW @532nm, 100mW @532nm * Yellow laser: 100mW @561nm * Orange laser: 50mW @594nm * High power UV-LED: 365nm Optics: Design and specifications may be subject to change due to further product development. Sysmex Europe GmbH, Bornbarch 1, 22848 Norderstedt, Germany, Phone: +49 (40) 527 26 0, Fax: +49 (40) 527 26 100 www.sysmex-e

* Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL6 * Exchangeable optical filters Flow System: * Quartz flow cuvette for laminar sample transport and hydrodynamic focussing * True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement * Computer controlled precision syringe pump for sample transport, speed continuously adjustable from 0-20 µ l/sec. Software: * Windows™ based FCM software CyView™ for real-time data acquisition, real-time data analysis and real-time data display * Easy experimental template set up (configuration...

* ~ 1h for 96-Well-Plate (speed 2 µl/sec., sample volume 20 µl) Software: * CyView™ windows based software CyFlow® Cube 8 Sorter General Facts: * Configurations with up to 5 optical parameters (up to 3 colours) * Sterile sorting by closed fluidic system * Optional CyFlow® Robby 8 Autoloading Station for well plates and tubes Light Sources: * Up to 2 simultaneous light sources (laser) * Blue laser: 50mW @488nm, 200mW adjustable @488nm * Red laser: 25mW @638nm, 40mW @640nm * Violet laser: 100mW @405nm * UV laser: 60mW @375nm * Green laser: 30mW @532nm, 100mW @532nm * Yellow laser: 100mW @561nm...

data display * Easy experimental template set up (configuration files) * Flow Cytometry Standard (FCS) data format for storage of original and evaluated data Dimensions: * L 500mm x W 470mm x H 370mm (without sorting module) Design and specifications may be subject to change due to further product development. Sysmex Europe GmbH, Bornbarch 1, 22848 Norderstedt, Germany, Phone: +49 (40) 527 26 0, Fax: +49 (40) 527 26 100 www.sysmex-europe.com