Gluten, a storage protein in wheat, rye, barley, and oats, is a common food allergen, with gliadin being the primary trigger for immune reactions in gluten-related disorders, which affect approximately 1–6% of the global population. The mainstay of treatment is adherence to a gluten-free diet, which is also being adopted by some with chronic diseases due to gluten's pro-inflammatory properties. This increasing awareness has heightened demand for gluten detection tests, especially considering the varying international gluten-free labeling standards (e.g., <20 ppm in the US, EU, UK, and Canada versus no detection levels in Australia and New Zealand). Analyte: Gliadin Method: Lateral Flow Immunoassay (LFIA) Limit of Detection (LOD): 10 ng/mL (10 ppb) Gliadin Allergen Rapid Test Rapid results within 5 minutes Accurate results with hook effect protection Sample Type: Solid, Liquid, Environmental (Surface Swab) Testing Time: 5 minutes Storage: Room temperature

Thoroughly clean your hands before operation to minimize the risk of gliadin contamination. Using the single-use dropper, add 20 drops (approximately 400 μL) of sample to the Extraction Tube. Tighten the cap and shake thoroughly to mix. Liquid Samples Let the sample sit in the Extraction Tube for one minute to allow complete lysis. Let the sample sit in the Extraction Tube for one minute to allow complete lysis. Interpret the result after 5 minutes. Do not interpret results after 15 minutes. Unscrew the dropper tip and dispense five drops (approximately 100 μL) into the sample well. Liquid Samples...

High Positive Very High Positive The appearance of the T line (T) together with the C line (C) and H line (H) indicates a positive result. Disappearance of the H line (H), or both the H line (H) and the line (T), may indicate an excessive amount of allergen protein in the sample. No colored line in the Control region (C) indicates an invalid test. A negative result is indicated when the T line (T) is absent and only the Cline (C) and H line (H) are visible. Result Interpretation Result Window A potential limitation of sandwich immunoassays is the hook effect (also known as the prozone effect),...