Cellartis 2i mES/iPSC Culture Medium: maintenance of germlinecompetent mouse ES and iPS cells
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Cellartis 2i mES/iPSC Culture Medium: maintenance of germlinecompetent mouse ES and iPS cells - 1

Cellartis 2i mES/iPSC Culture Medium: maintenance of germlinecompetent mouse ES and iPS cells Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors (2i technology: CHIR99021 and PD0325901) that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation.  More Cellartis® 2i mES/iPSC Culture Medium Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors ('2i'), CHIR99021 and PD0325901, that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation. Image Data Overview Completely defined, serum-free formulation promotes cell proliferation and maintains pluripotent state Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK, allowing establishment and long-term maintenance of germline-competent mouse ES and iPS cells Can be combined with Leukemia Inhibitory Factor (LIF) to induce the efficient conversion of partial- or pre-iPS mouse cells into fully pluripotent iPS cells More Information Applications Establishment of mouse ES and iPS cell lines Long-term maintenance of pure ES or iPS cells Conversion of partial- or pre-iPS cells into fully pluripotent cells in the presence of LIF Components 1 bottle (200 ml) of Cellartis mES/iPSC Culture Basal Medium 1 tube (200 µl) Cellartis 2i mES/iPSC Su

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Cellartis 2i mES/iPSC Culture Medium: maintenance of germlinecompetent mouse ES and iPS cells - 2

Malaver-Ortega, L. F. et al. The state of the art for pluripotent stem cells derivation in domestic ungulates. Theriogenology 78, 1749–1762 (2012). Nichols, J. et al. Validated germline-competent embryonic stem cell lines from non-obese diabetic mice. Nat. Med. 15, 814–818 (2009). Silva, J. et al. Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253 (2008). Silva, J. et al. Nanog is the gateway to the pluripotent ground state. Cell 138, 722–37 (2009). Wang, W. et al. Rapid and efficient reprogramming of somatic cells to induced pluripotent stem...

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