Streamlined single-cell mRNA-seq with the SMART-Seq HT Kit
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Streamlined single-cell mRNA-seq with the SMART-Seq HT Kit The core SMART-Seq HT Kit (SS-HT) and the SMART-Seq HT PLUS Kit (SS-HT PLUS) are automationfriendly kits that use oligo(dT) priming to generate high-quality, full-length cDNA directly from 1–100 cells or 10 pg–1 ng of total RNA. In addition to the core cDNA synthesis kit, the SS-HT PLUS kit also includes a library preparation kit and single-use unique dual index (UDI) plates to generate Illumina-compatible sequencing libraries, making it a complete end-to-end solution.  More SMART-Seq® HT Lysis Components The SMART-Seq HT Lysis Components are accessories to the SMART-Seq HT Kit. These components consist of 10X Lysis Buffer as well as the 3′ SMART-Seq CDS Primer II A, and are provided in separate packaging for users who want to use FACS to sort a large number of cells or nuclei without using all of the sorted material as input for cDNA synthesis with the SMART-Seq HT Kit. Image Data Overview Easily automate the streamlined workflow—process more samples in less time and maximize the space on your automation deck Unparalleled sensitivity—start with as little as one cell or 10 pg of total RNA (input range: 1–100 cells or 10 pg–1 ng of total RNA) High-quality RNA-seq data—capture full-length transcript coverage, a low percentage of rRNA r

Single-tube workflows—complete cDNA synthesis and library preparation, in one tube each, for minimization of sample loss, sample mixup, or other handling errors Increased sequencing power—include unique dual indexes to allow for the pooling of multiple samples and confident sequencing on the NovaSeq™ system High library yield—sequence on high-throughput sequencers with ease (such as the NovaSeq) or confidently save extra library for future runs Interested in more data and FAQs about this product? Visit the NGS Learning Center. SMART-Seq HT has a simplified workflow with less hands-on time...

Sequencing metrics comparing SMART-Seq v4 and SMART-Seq HT kits RNA source 10 pg Mouse Brain Total RNA cDNA synthesis Average Pearson/Spearman Proportion of reads mapped (%) rRNA Intergenic regions Table 1. High overlap of transcripts identified with the SMART-Seq v4 and SMART-Seq HT kits. Libraries were prepared from 10 pg of Mouse Brain Total RNA. The output cDNA was converted into RNA-seq libraries using the Illumina Nextera XT DNA Library Preparation Kit and sequenced on an Illumina NextSeq® instrument (2 x 75 bp). Sequences were analyzed as described in the methods after normalizing...

Figure 2. SMART-Seq HT PLUS generates more libraries and identifies more genes than Nextera XT. Panel A. SMART-Seq HT was used to produce cDNA from 10 pg of Mouse Brain Total RNA in triplicate. Illumina-compatible libraries were generated using SMART-Seq PLUS or Nextera XT (Illumina) library prep kits, and sequenced on a NextSeq 500 system. The reads were normalized to 4M paired-end reads and analyzed as described in the technical note. Panel B. Library yields obtained with SMART-Seq Library Prep Kit are higher than those generated with Nextera XT. Panel C. The SMART-Seq Library Prep Kit...