Prime Pro - 12 Pages

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Prime Pro

Catalog excerpts

400 qPCR detection kits Accurately quantify NGS libraries MIQE guidelines compliant High resolution melt Prime Pro 48 Real Time PCR System

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Introducing Prime Pro The Prime Pro 48 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5µl is validated, resulting in a...

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1. Load samples into 48 well plate using backlit loading block. The ergonomic plate loading dock allow for easy 2. Insert plate into Prime Pro 48. loading of the plate and is backlit to allow the user 3. Run a MIQE compliant experiment via ProControl software. to easily visualise sample loading. 4. Analyse results in ProStudy software. Base with LED backlight 5. Generate a full report automatically. Plate adapter fits standard centrifuge rotors Includes plate sealing tool Advanced optical technology Intuitive icons lead researchers through setup, run and analysis quickly and easily...

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Prime Pro 48 thermal system qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The unique thermal block design of Pro 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC temperature control and quickly cycles from one temperature to the next. Hollow pure silver block filled with conductive fluid, gold electroplated Peak paddles on titanium bearings Single...

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4 band pass filters (upgradable to 5) Thermal system The Prime Pro 48 Real Time PCR system contains an advanced high-performance optical system that delivers precise and sensitive fluorescence detection, facilitating all 4 colour multiplex applications. The system is provided factory-calibrated for use with SYBR®, FAMTM, HEXTM, VICTM, ROXTM, Cy®5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a...

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Applications The Prime Pro 48 is a high performance instrument for demanding applications. Genotyping and High resolution melting Absolute & Relative Quantitation • Allelic Discrimination. • Absolute quantification standard curves with efficiency calculation. • Relative quantification using the ΔΔCq method with • Microbiology: pathogen detection and identification. support for multiple reference gene normalization. • Relative gene expression with efficiency control. • Next Generation Sequencing (NGS) library quantification. • Copy Number Variation: insertions, deletions, inversions. • RNA...

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Library quantification • qPCR is the most precise method for quantifying • Academic, Government and Corporate laboratories. libraries prior to cluster generation. • Human research, AgriGenomics, Translational, Forensics • Works at concentrations below the detection threshold of conventional spectrophotometric • Probe, DNA Binding Dye and HRM chemistries. • Gene expression, Viral detection, Sequencing Library and many more markets. Preparation, Genotyping and Mutation Screening • Validation of array and sequencing quality data. • Pro 48 is for research use only – not suitable for human or...

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Rapid cycling The Pro 48 has rapid heating and cooling rates. Average ramp rates of 5.5°C/sec reduce overall PCR run times. The typical run time for a 40 cycle PCR protocol is under 20 minutes when optimised. Pro 48 hollow silver fast block, gold plated High throughput Pro 48 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier increases. With Pro 48, faster and more precise cycling increases sample throughput because the need for multiple replicates is reduced by increased uniformity across the block. Pro 48...

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Software Easy to use software that integrates user control, real-time data collection, and advanced data analysis. Data Analysis • User-installable, intuitive, easy to use software that • With the Pro 48 system and software, data collection integrates user control, real-time data collection, and is monitored in real time, allowing researchers to access advanced data analysis. • Supplied fully featured including HRM capability. • The user-friendly data analysis interface also allows • New software version 5 enables a widened chemistry researchers to easily view the amplification plot, melt...

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qPCR probe-based detection reagent kits Every assay is expert designed, involving in-depth literature review followed by extensive bioinformatics analysis. The kits are optimised via a strict biochemical validation on synthetic oligos to ensure optimal PCR performance. Every kit operates with the same standardised protocol and cycling conditions, which allows multiple kits to be used on the same plate in the same PCR run. • Designed and manufactured in the UK. • Wide range of 400 qPCR detection kits. • Designed to work with Pro 48 and all other qPCR machines. • Kits sold with/without...

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Prime Pro 48 technical specification High Resolution Melt Detection sensitivity Temp uniformity Temperature range Average ramp rate Thermal system Proprietary hollow silver block, Peltier-based system with conductive fluid Block format 48-well custom plates and optical adhesive seals Optical system Dual LED excitation (452–486 nm and 542–582 nm). CCD camera Calibrated dyes SYBR®, FAMTM, HEXTM, ROXTM, Cy®5. Additional dyes within the wavelength range compatible with Pro 48 filters are supported with no additional calibration Passive reference dyes Use of ROXTM is supported, but optional Data...

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