Intended Use For the quantitative determination of direct and total bilirubin in human serum. Principle Bilirubin reacts with the diazotized sulfanilic acid to produce azobilirubin, which has an absorbance maximum at 560 nm in the aqueous solution. The intensity of the color produced is directly proportional to the amount of direct bilirubin concentration present in the sample. The subsequent addition of methanol accelerates the reaction of unconjugated bilirubin in the serum, and a value for total bilirubin is obtained. The total bilirubin value represents the sum of the direct and indirect bilirubin. The color produced measured at 560 nm is proportional to the amount of the total bilirubin concentration present. CONTACT US: TECO DIAGNOSTICS 1268 N. Lakeview Avenue Anaheim, CA 92807 Tel: 714‐463‐1111 Fax: 714‐463‐1169 Test: Direct & Total Bilirubin (B577‐480) Number of Tests: 480 tests 4 x 120 mL bottles Format: Liquid Method: Colorimetric, Endpoint Testing Procedure: Manual Storage Temperature: 15‐30°C Wavelength: 560 nm Linearity: 20 mg/dL Expected Values: Direct: Up to 0.5 mg/dL Total: Up to 1.0 mg/dL It is strongly suggested that each laboratory establish its own range of expected values. Reagent Deterioration: The reagent should be discarded if: (1) Sodium Nitrite reagent has a yellow discoloration; (2) Reagent fails to achieve assigned assay values of fresh control sera.