C509-400
1Pages

Intended Use For the quantitative determination of total cholesterol in human serum. Principle Cholesterol esters are hydrolyzed to produce cholesterol. Hydrogen peroxide is then produced from the oxidation of cholesterol by cholesterol oxidase. In a coupled reaction catalyzed by peroxidase, quinoeimine dye colored red is formed from 4‐ aminoantipyrine, p‐HBS, and hydrogen peroxide. The absorption at 520 nm of the solution of this dye is proportional to the concentration of cholesterol in the sample. CONTACT US: TECO DIAGNOSTICS 1268 N. Lakeview Avenue Anaheim, CA 92807 Tel: 714‐463‐1111 Fax: 714‐463‐1169 Test: Cholesterol Reagent Set (Phenol Free) (C509‐400) Number of Tests: 400 tests 8 x 50 mL bottles Format: Powder Method: Colorimetric Testing Procedure: Manual Storage Temperature: 2‐8°C Reconstituted Stability: 60 days at 2‐8°C( in an amber bottle) Wavelength: 520 nm Linearity: 500 mg/dL Expected Values: Desirable: < 200 mg/dL Borderline high: 200‐239 mg/dL High: ≥240 mg/dL It is strongly suggested that each laboratory establish its own range of expected values. Reagent Deterioration: The reagent should be discarded if: (1) Turbidity has occurred; (2) Moisture has penetrated the vial and caking is observed; (3) The reagent fails to meet linearity claims or fails to recover control values in the stated range. Limitations of Procedure: Anticoagulants such as fluoride and oxalate will res