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C509-400

C509-400
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C509-400

Product catalog summary
Intended Use: This document outlines the use of a reagent set for the quantitative determination of total cholesterol in human serum.
Principle: The method involves hydrolyzing cholesterol esters to produce cholesterol, which is then oxidized to produce hydrogen peroxide. A coupled reaction catalyzed by peroxidase forms a red quinoeimine dye, with its absorption at 520 nm being proportional to the cholesterol concentration.
Contact Information: TECO DIAGNOSTICS, 1268 N. Lakeview Avenue, Anaheim, CA 92807. Tel: 714-463-1111, Fax: 714-463-1169.
Test Details: The Cholesterol Reagent Set (Phenol Free) is identified as C509-400, capable of performing 400 tests with 8 x 50 mL bottles. The format is powder, and the method is colorimetric with manual testing procedures.
Storage and Stability: Store at 2-8°C. Reconstituted stability is 60 days at 2-8°C in an amber bottle.
Wavelength and Linearity: The wavelength for measurement is 520 nm, with linearity up to 500 mg/dL.
Expected Values: Desirable cholesterol levels are < 200 mg/dL, borderline high is 200-239 mg/dL, and high is ≥240 mg/dL. Laboratories should establish their own expected value ranges.
Reagent Deterioration: Discard the reagent if turbidity occurs, moisture causes caking, or if it fails to meet linearity claims or recover control values.
Limitations: Anticoagulants like fluoride and oxalate may cause false low values. The test is not affected by hemoglobin up to 200 mg/dL or bilirubin up to 10 mg/dL. Interference from icteric and hemolyzed samples can be corrected with a serum blank.
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Catalog excerpts

C509-400-1

Intended Use For the quantitative determination of total cholesterol in human serum. Principle Cholesterol esters are hydrolyzed to produce cholesterol. Hydrogen peroxide is then produced from the oxidation of cholesterol by cholesterol oxidase. In a coupled reaction catalyzed by peroxidase, quinoeimine dye colored red is formed from 4‐ aminoantipyrine, p‐HBS, and hydrogen peroxide. The absorption at 520 nm of the solution of this dye is proportional to the concentration of cholesterol in the sample. CONTACT US: TECO DIAGNOSTICS 1268 N. Lakeview Avenue Anaheim, CA 92807 Tel: 714‐463‐1111 Fax: 714‐463‐1169 Test: Cholesterol Reagent Set (Phenol Free) (C509‐400) Number of Tests: 400 tests 8 x 50 mL bottles Format: Powder Method: Colorimetric Testing Procedure: Manual Storage Temperature: 2‐8°C Reconstituted Stability: 60 days at 2‐8°C( in an amber bottle) Wavelength: 520 nm Linearity: 500 mg/dL Expected Values: Desirable: < 200 mg/dL Borderline high: 200‐239 mg/dL High: ≥240 mg/dL It is strongly suggested that each laboratory establish its own range of expected values. Reagent Deterioration: The reagent should be discarded if: (1) Turbidity has occurred; (2) Moisture has penetrated the vial and caking is observed; (3) The reagent fails to meet linearity claims or fails to recover control values in the stated range. Limitations of Procedure: Anticoagulants such as fluoride and oxalate will res

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