Cytomegalovirus
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INFECTIOUS SEROLOGY – VIROLOGY – CYTOMEGALOVIRUS ELISA, IMMUNOBLOT and MICROBLOT-ARRAY kits are optimized and validated for detection of IgA, IgG and IgM antibodies in human serum, plasma or cerebrospinal fluid Diagnostic kits are intended for professional use in the laboratory. Enzyme immunoassays for the diagnostics of cytomegalovirus infectio

INFECTIOUS SEROLOGY – VIROLOGY – CYTOMEGALOVIRUS Diagnostic significance of antibody classes Human cytomegalovirus (CMV, Human Herpesvirus 5, IgA: Antibodies of IgA class are a sign of an active infec- HHV 5) is a member of the Herpetoviridae family. Primary infection of CMV occurs mainly in childhood or adolescence. The infection can be transmitted in different ways (e.g. respiratory apparatus, digestive or urogenital tract). Clinically, the disease process is usually tion – primary infection as well as reactivation. The IgA antibodies are produced during reactivation and they can rarely...

Results interpretation IgA IgM IgG Avidity Interpretation + + - non done Acute infection + (+) + high Convalescence or reactivation - - + high Past infection Description Tegument protein UL32 A strong immunogen of the late stage of infection (late antigen); it does not develop in the early stage Detectable in the IgG class in higher titres even in reactivation Immediate early antigen, capsid protein UL123 Plays a role in the early phase of the replication cycle of human CMV Important function in defence mechanisms against CMV infection Tegument protein UL83 In the IgM class - one of the...

infectious serology - virology - cytomegalovirus elisa Test principle The assays are based on a sandwich type ELISA method. ^HRp) ^HRp) ^^rnti-Hu IgG ^^nti-Hu IgM | Anti-Hu IgA ^^gG IgM Antigens Purified and inactivated antigen isolated from AD 169 strain of Cytomegalovirus enriched with highly specific immunodominant epitopes. Microtiter plate Summary protocolStep Test steps Dilution of samples J 1. - serum/plasma 1:101 (io pl + 1 ml) - cerebrospinal fluid 1:3 (50 pl + 100 pl) Pipette Controls and diluted samples £ 2. 100 mI - Including blank Q 3. Incubate 30 min. at 37 °C © 4. Aspirate...

IMMUNOBLOT Test principle Summary protocol Recombinant antigens are transferred to a nitrocellulose membrane using a micro-dispensing method. Reaction of substrate and enzyme resulting in coloured insoluble product Strips soaking 10 min. at room 2. Enzyme-conjugated secondary antibody binding to primary antibody Specific primary antibody binding to protein Blocking agent temperature – Shaker Specific antigen protein Pipette Universal solution 2.5 ml Aspirate Dilute samples – serum/plasma 1:51 (30 μl + 1,5 ml) Pipette Controls and diluted samples 1.5 ml Incubate 30 min. at room temperature –...

User comfort - Ready-to-use components - Colour-coded strips - Interchangeable components - Positive and Negative controls - Control lineis present on the strip - Possibility of software evaluation Clinical application - Detailed determination of the presence of antibodies against specific CMV antigens - Confirmation of ambiguous results - Confirmatory to ELISA tests Test characteristics Immunoblot Sensitivity Specificity Advantages - Identical assay procedure - Easy interpretation and reproducibility of results - Sophisticated evaluation software - High diagnostic specificity and...

microblot-arrayDistribution of antigens and control spots Protocol Summary Step Test steps ^ 1. Pipette Universal solution 150 pl Strips soaking 10 min. at room Description of control spots O r - Reference O tc - Test control O CM - Conjugate control IgM O CG - Conjugate control IgG O C1 - Calibration 1 O C2 - Calibration 2 O C3 - Calibration 3 O C4 - Calibration 4 User Comfort - Low sample consumption - Antigens spotted in triplicate - minimizing statistical variation - Possibility of automatic assay processing and results evaluation - Parallel testing of multiple markers simultaneously -...

infectious serology - virology - cytomegalovirus Ordering information elisa Cat. No. Product SmartEIA kits are designed for automated processing using the Agility® analyser. Units 96 wells 96 wells 96 wells 96 wells 96 wells 96 wells 3,5 ml 3,5 ml microblot-array Kod Product Units il Test Line® TestLine Clinical Diagnostics Ltd. Krizikova 68, 612 00 Brno, Czech Republic +420549 121 203 sales@testlinecd.com www,testlinecd,com Company is certified to the quality management system standards ISO 9001 and ISO 13485 for in vitro diagnostics,