Microblot-Array Multiplex diagnostics in microtiter plate format

Definition of efficient multiplex diagnostics Microblot-Array is an immunoblot array in microtiter plate format designed for efficient multiplex diagnostics. The technology eliminates the bottleneck of traditional BLOT processing and capacity and opens up the way to high throughput testing and automation. The comprehensive evaluation of Microblot Array testing is ensured by using the Microblot-Array Software in combination with the Microblot-Array Reader, enabling complex image analysis including results evaluation and connectivity to LIS. Main clinical areas covered – Infectious serology – Autoimmunity...

Complex solution Microblot-Array Software Calibration data – Automated test identification – Intuitive and user-friendly guiding throughout the results evaluation evaluation with LOT identification – Calibration data ensure significant – Complex image analysis – Optional manual control of spot localization – Detailed results comparison within single wells and substrate between the same – Evaluation of the validity test through control spots – Unification of evaluation criteria – Export of results in various formats – The more effective automatic processing Microblot-Array – Antigens spotted in...

Benefits Efficiency – Analysis of up to 96 patient samples per plate – Possibility of automated processing using an – Low sample consumption – 10 μl – Parallel testing of multiple markers simultaneously – Time and cost saving diagnostics ELISA instrument – Intuitive software for test evaluation – Evaluation of individual antigens and their association with pathogen species or disease type User comfort – One parameter × various parameters – Ready-to-use components – One well × high number of samples – Identical assay procedure (30–30–15 min.) – Manual processing × automated processing – Remote...

Protocol Summary Step No. Pipette Universal Solution – 150 µl Wells soaking at room temperature for 10 min. Dilute samples serum/plasma 1:51 (10 µl + 500 µl) cerebrospinal fluid 1:3 (50 µl + 100 µl) synovial fluid 1:17.5 (10 µl + 165 µl) Pipette control and diluted samples – 100 µl Incubate at room temperature for 30 min. Quick wash using the Universal Solution Aspirate and wash 3 × 5 min. with 150 µl of Universal Solution Incubate at room temperature for 30 min. Quick wash using the Universal Solution Aspirate and wash 3 × 5 min. with 150 µl of Universal Solution Pipette Substrate Solution (BCIP/NBT)...

Microblot-Array for the diagnostics of systemic autoimmune diseases The main benefit of Microblot-Array ANA kits is the high number of antigens which can be simultaneously detected in one sample. The kits are primarily intended for confirmation of ELISA or other screening method. However, they also enable identification of specific antibody and thus differentiation of systemic autoimmune diseases, such as myositis, scleroderma, systemic lupus and others. The kits are optimized and validated for detection of specific IgG in human serum or plasma. Test Characteristics Parameters of the Microblot-Array...

Hystidyl tRNA synthetase Threonyl tRNA synthetase Alanyl tRNA synthetase Glycyl tRNA Synthetase Isoleucyl tRNA synthetase Asparaginyl tRNA synthetase Tyrosyl tRNA synthetase (Ha) Phenylalanyl tRNA synthetase Phenylalanyl tRNA synthetase Small ubiquitin-like modifier activating enzyme Small ubiquitin-like modifier activating enzyme Signal recognition particle Helicase protein-nuclear transcription Transcription Intermediary Factor 1 Melanoma differentiation associated protein 5 (CADM-140) Nuclear matrix protein 2 (p140, MJ) Human exosome complex Human exosome complex Nucleolar transcription factor...

Microblot-array for the diagnostics of Bordetella pertussis and Bordetella parapertussis Microblot-Array Bordetella kits provide the detailed determination of the presence of specific IgA, IgG, and IgM antibodies to recombinant Bordetella pertussis and Bordetella parapertussis antigens in human serum or plasma. It can be used for differentiation of postinfection and postvaccination antibodies as well as for differentiation disease stage. It confirms positive or borderline ELISA or agglutination test. Test Characteristics Parameters of Microblot-Array Bordetella kits Pathogen Diagnostic Sensitivity...

Spot No. Antigen Description Pertussis toxin (45 kDa) - basic virulence factor, specific only for B. pertussis, the most important pertussis antigen B. pertussis filamentous hemagglutinin - adhesive protein, FHA important immunogen; selected part of the sequence with high specificity ^ Adenylate cyclase toxin (CyaA) - significant virulence factor of B. pertussis with anti-phagocytic activity Tracheal colonization factor - protein produced only by B. pertussis; adhesin; enabling the microorganism to adhere to mucosal surfaces of respiratory tract and colonize ciliated epithelial cells and phagocytes...

Microblot-Array for the diagnostics of Borrelia species and Anaplasma phagocytophilum @ (©©) (©(©(©(©(©(©©) ©) (© ©@@©©©©© (£3) (to A1 A2 A3 A4©(A6 A^ (A^ © ©@®©®©(S S ©© A1 £ ©©©©(g A8 b) The kits are optimized for the detection of specific IgG and IgM antibodies to recombinant antigens of Borrelia species and Anaplasma phagocytophilum (HGA) in human serum, plasma, cerebrospinal or synovial fluid. Serological diagnostics of borreliosis is difficult due to the large genetic diversity of the species Borrelia burgdorferi s.l., possible cross reactivity with unrelated antigens of other microorganisms...

(Ba - B. afzelii, Bg - B. garinii, Bs - B. burgdorferi sensu stricto, Bsp - B. spielmanii)