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Parvovirus B19

Product catalog summary

Introduction
Parvovirus B19, a member of the Parvoviridae family, is a common human pathogen with humans as its sole host. It is a non-enveloped, single-stranded DNA virus composed mainly of the VP2 protein. Infection can occur year-round, often transmitted through direct contact, blood derivatives, or vertically from mother to fetus. While most infections are asymptomatic, they can cause mild respiratory symptoms or more severe conditions like aplastic crisis in individuals with hemolytic diseases. In pregnant women, the virus poses a risk of severe fetal anemia.

Diagnosis of Infection
Diagnosis involves detecting IgM and IgG antibodies. IgM antibodies appear around 10 days post-infection, peaking in 2-3 weeks and lasting up to 3 months. IgG antibodies develop shortly after and persist for life. The prevalence of antibodies increases with age, ranging from 50% to 85% in adults.

ELISA Test Principle
The ELISA kits use a sandwich method to detect IgG and IgM antibodies in human serum or plasma. The process involves sample dilution, incubation, washing, and colorimetric detection at 450 nm.

Clinical Application

Screening for specific IgG and IgM antibodies.
Monitoring therapy results through semiquantitative determination.
Diagnosing disease stages and differentiating exanthematous diseases.

User Comfort and Advantages

Ready-to-use, color-coded components.
High diagnostic specificity and sensitivity.
Short assay time and high reproducibility.
Automation-ready and independent verification possible.

Test Characteristics
The diagnostic sensitivity and specificity for IgG are 98.6% and 99.9%, respectively, and for IgM, 96.8% and 99.9%.

Ordering Information
ELISA kits are available for both IgG and IgM detection, with options for automated processing using the Agility® analyser. The company is certified under ISO 9001 and ISO 13485 standards for in vitro diagnostics.

Catalog excerpts

INFECTIOUS SEROLOGY – VIROLOGY – PARVOVIRUS B19 Enzyme immunoassays for the diagnostics of Parvovirus B19 infection Diagnostic kits are intended for professional use in the laboratory. ELISA kits are optimized and validated for detection of IgG and IgM antibodies in human serum or plasm

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INFECTIOUS SEROLOGY – VIROLOGY – PARVOVIRUS Introduction Parvovirus B19 (Erythrovirus B19) belongs the Par- infection during pregnancy also represents a severe voviridae family. It is a common human pathogen. Man risk. Transplacental transmission of infection occurs is its sole host. It is a non-enveloped single-stranded approximately in 1/3 of infected pregnant women and DNA virus. It consists of two structural proteins, VP1 may have fatal consequences due to severe anaemia of and VP2, whereas VP2 is the major one and it forms the foetus (hydrops of the foetus, risk of death of the approximately...

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ELISA Test principle The assays are based on a sandwich type of ELISA method. Antigens VP2 recombinant protein Microtiter plate Clinical application - Screening test for the detection of specific IgG and IgM antibodies in human serum or plasma - Checking of therapy results using the semiquantitative determination - Disease stage diagnosis - Differential diagnosis of exanthematous diseases 1 Dilution of samples - serum/plasma 1:101 (10 pl + 1 ml) Pipette Controls and diluted 2. samples 100 pl - Including blank User comfort - Ready-to-use components; colour-coded components and interchangeable...

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INFECTIOUS SEROLOGY - VIROLOGY - PARVOVIRUS Ordering Information ELISA Cat. No. Product No. of Tests SmartEIA kits are designed for automated processing using the Agility® analyser. il Test Line® TestLine Clinical Diagnostics Ltd. Krizikova 68, 612 00 Brno, Czech Republic +420549 121 203 [email protected] www.testlinecd.com Company is certified to the quality management system standards ISO 9001 and ISO 13485 for in vitro diagnostics.

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Parvovirus B19

Parvovirus B19

Catalog excerpts

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