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Human Health Diagnostic Products

Human Health Diagnostic Products

Human Health Diagnostic Products

Product catalog summary
Overview: The document provides detailed information on various molecular genetics products, specifically focusing on PCR kits used for human health diagnostics. These kits are categorized into conventional PCR kits, hydrolysis real-time PCR kits, hybridization real-time PCR kits, and cytogenetic and FISH products.
Conventional PCR Kits: The AZOplex® IV AZF Detection PCR Kit is highlighted for its role in identifying Y chromosome microdeletions, crucial for male infertility studies. It uses multiplex PCR amplification to detect 14 microdeletions in AZF regions and the SRY region. The PCR products are visualized using agarose gel electrophoresis.
Hydrolysis Real-Time PCR Kits: These kits utilize probes and primers with 5' nuclease activity of Taq DNA polymerase. The document lists several kits, including those for detecting Factor II (Prothrombin G20210A), Factor V Leiden (G1691A), and MTHFR mutations. The kits are optimized for use with specific real-time PCR devices and require whole blood specimens.
Hybridization Real-Time PCR Kits: These kits use primers and hybridization probes designed for specific gene regions. The document details kits for detecting various genetic mutations, including Factor II, Factor V Leiden, and Jak2 (V617F). The kits are optimized for use with Roche Applied Science LightCycler 480 and Rotor-gene Q devices.
Cytogenetic and FISH Products: The document outlines various probe kits, including centromere-specific, locus-specific, and sex chromosome probe kits. These kits are designed for easy application and include counterstains like DAPI.
Key Specifications: Each kit is listed with its catalog number, pack size, and necessary equipment for extraction and amplification. The document emphasizes the importance of using the specified equipment and conditions for optimal results.
Conclusion: The document serves as a comprehensive guide for selecting and using PCR kits for molecular genetics diagnostics, providing essential details on the application, analysis, and equipment required for each product.
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Catalog excerpts

Human Health Diagnostic Products-1

Molecular Genetics Products Conventional PCR Kits • AZOplex® IV AZF Detection PCR Kit Hydrolysis Real Time PCR Kits • Factor II (Prothrombin G20210A) Real Time PCR Kit • Factor V Leiden (G1691A) Real Time PCR Kit • MTHFR (C677T) Real Time PCR Kit • MTHFR (A1298C) Real Time PCR Kit. • FXIII (Val34Leu) Real Time PCR Kit • HLA-B27 (Ankylosing spondylitis) Real Time PCR Kit Hybridisation Real Time PCR Kits • Factor II (Prothrombin G20210A) Real Time PCR Kit • Factor V Leiden (G1691A) Real Time PCR Kit • MTHFR (A1298C) Real Time PCR Kit • MTHFR (C677T) Real Time PCR Kit • PAI-1 (4G/5G) Real Time PCR Kit • FXIII (Val34Leu) Real Time PCR Kit • FMF (M694V, M694I, M680(G/C), V726A, K695R) Real Time PCR Kit Cytogenetic and FISH Products • Centromere Specific Probe Kits • Locus Specific Probe Kits • Cancer Probes

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Human Health Diagnostic Products-2

AZOplex® IV AZF Detection PCR Kit (Chromosome Y Microdeletion PCR Kit) Identification and analysis of Y chromosome microdeletions play an important role in male infertility studies. With the discovery of PCR and the identification of microdeletions of STS regions in the Y chromosome, AZF microdeletions were identified in patients. AZOplex® IV AZF kit is used to identify 14 microdeletions in all AZF regions and the SRY region on Y chromosome. 15 different primer sequences were combined in 4 groups. The presence or absence of all 15 target regions using a single PCR protocol is determined by multiplex...

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Human Health Diagnostic Products-3

Hydrolysis Real-time PCR Kits Test Principle Polymerase Chain Reaction (PCR) is a method using probes and primers with 5 'nuclease activity of Taq DNA polymerase. The probes are composed of a 5 'end reporter and a 3' end quencer consisting of fluorescent dyes. The hybridization of probes and primers to the target gene region increases the amount of fluorescent light as a result of the probe breaking down and emitting signal. The signal increase shows amplification. Analysis of PCR Products PCR products are interpreted according to the Heterozygote Mutant control DNA contained in the kit as normal, heterozygous...

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Human Health Diagnostic Products-4

Hybridisation Real-time PCR Kits Test Principle Polymerase Chain Reaction (PCR) method using primers and hybridization probes. Hybridization probes are designed specifically for the gene region of interest. The target gene region is amplified with primers. In the post PCR process, the amount of light in the hybridization probes increases or decreases depending on the melting temperatures of the peaks determined in the protocol. Interpretation of this situation can determine the change in the course. Analysis of PCR Products PCR products are interpreted according to the Heterozygote Mutant control...

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Human Health Diagnostic Products-5

Probe KitsGeneral Probe Categories; and 11 are supplied different color combination) supplied different color combination) • Centromere specific probe kits • Combination of spesific probe kits (i.e. Chromosomes 8 • Locus-specific probe kits • Sex chromosome probe kits (Chromosomes X and Y are • All centromeric probe kits • All telomeric probe kits Antifade and DAPI counterstain vials Kit Features; • Ready to use kit companents • Easily applicableDifferent colors of probes (Green, Red, Aqua and required other colors) • Included counterstain (DAPI or required PI) Descriptions Chr 1 Centromeric...

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