2012-2013 Thermo Scientific Chromatography Columns and Consumables Trusted solutions uncompromised analysis

Thermo Scientific Chromatography Columns and Consumables 2012-2013 Sample Preparation Products Our comprehensive range of sample preparation products have been developed for rapid, effective and economical sample preparation. Thermo Scientific HyperSep SPE products are available in a range of formats including columns, multi-well plates and pipette tips. We also offer the complete line of Thermo Scientific syringe filters and the innovative WebSeal™ system.

SPE Column Selection . . . . . . . . . . 1-002 SPE Procedure . . . . . . . . . . . . . . . . 1-005 SOLA SPE Columns and Plates . . . 1-007 SPE Columns and Plates . . . . . . . . 1-012 SPE Accessories. . . . . . . . . . . . . . . 1-037 eVol Dispensing System . . . . . . . . 1-053 QuEChERS Products . . . . . . . . . . . . 1-057 Syringe Filters . . . . . . . . . . . . . . . . 1-064 WebSeal. . . . . . . . . . . . . . . . . . . . . 1-074 Automated SPE for Organic Pollutants . . . . . . . . . . 1-080 HyperSep Retain First in class SPE performance, maximize reproducibility and extract cleanliness Reproducible...

Thermo Scientific SPE Products Thermo Scientific HyperSep SPE products have been developed to meet the requirements of today's sample preparation challenges. HyperSep SPE columns and multi-well plates are offered in a range of phases, ideal for use in application areas such as: Pharmaceutical, Biochemical, Environmental, and Food and Beverage. The following flow chart is designed to help in selecting the correct phase for the compound of interest. Reversed Phase HyperSep Sorbent(s) Moderately Polar Reversed Phase Reversed Phase SOLA, Hypercarb Non-Polar & Cationic Non-Polar & Anionic Reversed...

Thermo Scientific HyperSep Product Alternative To CLEAN-UP C18-U Supelclean™ ENVI-18 / LC-18 SampliQ C18 BAKERBOND spe™ Polar Plus™ Isolute™ C18 CHROMABOND™ C18 Bond Elut™ C18 strata™ C18-U Sep-Pak™ C18 CLEAN-UP C8 Supelclean ENVI-8 / LC-8 SampliQ C8 BAKERBOND spe Octyl C8 Isolute C8 CHROMABOND C8 Bond Elut C8 strata C8 Sep Pak C8 HyperSep Phenyl Page 1-027 HyperSep Silica Page 1-028 HyperSep Aminopropyl Page 1-034 HyperSep Florisil Page 1-033 CLEAN-UP Phenyl Supelclean LC-Ph SampliQ Phenyl BAKERBOND spe Phenyl Isolute Ph Bond Elut Ph strata Phenyl (PH) CLEAN-UP Silica Supelclean LC-Si SampliQ...

Sample Preparation Removing Uncertainty by Applying the Science to SPE Our comprehensive range of SPE solutions offer un-paralleled performance in purity of extract and reproducibility. Having a fundamental effect on the quality, time and analysis cost, SPE is a critical step during the sample analysis procedure. We are dedicated to supplying the highest quality SPE solutions, in combination with providing our customers with the support and resources to optimize their SPE solutions and maximize their analysis. The Importance of SPE Reducing the effects of the matrix on the separation (GC/LC)...

3. Column Pre-equilibration 1. Sample Pre-treatment 5. Wash Away Interferences Equilibrate with weakly eluting solvent to prepare the phase for sample addition. Remove impurities bound less strongly than the compounds of interest. Use the same solvent as for sample pre-treatment Prevent sorbent drying during column equilibration 4. Sample Loading 3. Column Pre-equilibration 5. Wash Away Interferences 6. Elute Compounds of Interest 1. Sample Pre-treatment It is important to optimize the sample for effective analyte retention. The following should be considered: Sample volume/analyte concentration/...

Sample Preparation Method Development Optimization in SPE For cleaner extracts, optimization of the SPE process can be important. By optimizing the load, wash, and elution steps of the SPE process, a cleaner sample extract can be obtained. An example of this is in the development of an SPE method for testosterone. HyperSep Retain PEP Using a 30mg 1mL SPE Cartridge (Part Number 60107-201) 1. Condition with 1mL methanol followed by 1mL water 2. Load 1mL of 500ng/mL sample in water 3. Sequentially wash with increasing strengths of methanol in water, collecting the eluent 0% methanol/100% water to...

Join the revolution Thermo Scientific SOLA products revolutionize Solid Phase Extraction (SPE). This first fritless SPE product range provides greater reproducibility with cleaner, more consistent extracts. SOLA products provide unparalleled performance characteristics compared to conventional SPE, phospholipid removal and protein precipitation products. This includes: Higher levels of reproducibility Higher levels of extract cleanliness Reduced solvent requirements Increased sensitivity SOLA products provide reduced failure rates, higher analysis speeds and lower solvent requirements which are...

Figure 2 shows that SOLA products have consistent recoveries (across all 30 test samples). The conventional loose packed SPE product from competitor (i) shows that on average one in every four samples gives a significantly lower recovery. This results in inconsistencies in results. In comparison SOLA products provide significantly higher levels of reproducibility which is vitally important for high throughput studies. 120 Caffeine SOLA Competitor (i) Sample Preparation Improved reproducibility Figure 2: Shows inconsistency of loose packed products compared to SOLA. Figure 3 shows that SOLA products...

SOLA Competitor (i) Competitor (ii) SOLA SPE Columns and Plates The data in Figure 4 shows significantly higher recovery levels and lower variability sample to sample with SOLA products at low elution volumes compared to conventional loose packed SPE products. This ensures correct results time after time. Elution Volume Carbamazepine Figure 4: SOLA products recovery and reproducibility at low extraction volumes

SOLA products proprietary manufacturing process provides a cleaner product and as a result a cleaner sample extract. This is shown in Figure 5 where SOLA products are compared against competitor (i) conventional loose packed SPE product, which have both been extracted with acetonitrile, dichloromethane and methanol, respectively. 100 90 80 Relative Abundance Sample Preparation Methanol extract comparison: SOLA products versus competitor (i) Relative Abundance Acetonitrile extract comparison: SOLA products versus competitor (i) Relative Abundance Thermo Scientific Chromatography Columns and Consumables...