Bonded Silica-Based Columns for HPLC Thermo Scientific brand

Ordering and Warranty Information In the U.S. Acclaim 120 Acclaim 300 Acclaim PA/PA2 Acclaim OA All Orders Including: • Credit Card Orders (VISA, American Express, MasterCard) • Blanket Orders • Emergency Orders Call 1-800-Dionex-0 (1-800-346-6390) or Acclaim 120 Acclaim PA Acclaim Explosives FAX 1-408-736-4476 Ordering by Credit Card Dionex accepts payment by MasterCard, VISA, and American Express cards with minimum orders of $50.00. Call 1-800-DIONEX-0 (1-800-346-6390) for more information. Blanket Orders Acclaim OA Acclaim PA/PA2 Acclaim 120 Dionex will arrange for blanket orders with a specified...

Introduction INTRODUCTION Par­ I: Introduction t Acclaim Column Families at a Glance Dionex Acclaim columns are silica-based columns designed for high efficiency separations and manufactured using ultra-high purity silica. Product families consists of a broad spectrum of stationary phases in the form of the conventional C8 and C18 phases. polar embedded phases. and several specialty phases, each designed for a specific application or group of applications. All Acclaim columns are designed and manufactured at Dionex, to tight specifications, to ensure consistent performance. General Purpose HPLC...

Dionex phase Bonded phase Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica Particle shape Particle size Average pore diameter Total carbon content Acclaim General Purpose Column Specifications Acclaim Specialty Column Specifications Specialty HPLC Columns Dionex phase Acclaim Surfactant Bonded phase na na Specialty column for fast organic acid analysis Yes Yes Endcapped Substrate Particle shape Particle size The Acclaim OA column features a patented polar-embedded stationary phase optimized and use tested for hydrophilic Ultrapure silica Ultrapure OA Ultrapure...

Introduction INTRODUCTION Selecting an Acclaim Column There are four questions to consider when choosing an HPLC column: 1. What are the goals of the separation? • Speed of analysis • Critical peaks requiring resolution strongly hydrophobic molecules and this will be the only retention mechanism. As the density of the bonded phase decreases, other retention mechanisms will come into play, such as ion exchange interactions between the sample and residual silanol groups on the surface of the silica gel, or sieving effects for similar sized molecules of different shapes. Thus, these studies can...

Introduction INTRODUCTION Acclaim Selectivity Column: Mobile Phase: Temperature: Flow Rate: 4.6 × 150 mm 49/51 w/w methanol/phosphate buffer, pH 7.0 40 °C 1 mL/min Column: Mobile Phase: Temperature: Flow Rate: Ethyl benzoate Dimethylaniline 0.4 Phenol Aniline Pyridine Retention Time Relative to Toluene Relative Retention Time vs Toluene o-Terphenyl 2 Ethylbenzene Toluene Hydrophobic Compounds Probe Molecules Property Indicator Hydrophobic interaction Selectivity for molecular shape Phenol, aniline and pyridine Dipole-dipole interactions and hydrogen bonding Choosing the Appropriate Particle Size...

Introduction INTRODUCTION Understanding More about the Bonded Phase Column Diameter Dionex offers highest quality silica-based reversed phase packing materials. The type of bonding, carbon load and endcapping are all factors that contribute to the superior bonded phase. Type of Bonding Functional groups are attached to the silica substrate either via a single attachment (monomeric bonding), or via multiple attachments (multidentate bonding). Monomeric bonded phases provide higher column efficiencies, than polymeric phases, but polymeric phases are very stable under pH extremes. Select a column...

The effect of mobile phase composition on column lifetime should be part of the consideration for designing a method. Use the highest practical quality of water, solvent, and buffer components; HPLC-grade material has low UV absorbance and is submicron-filtered by the manufacturer. To prevent fouling of your Acclaim column, use mobile phases that have been filtered through a 0.5-µm or smaller filter. Both alkaline conditions and strong acid conditions will degrade silica-based columns over time. The silica substrate resists acids, but is soluble in alkali. The upper pH limit is determined by...

Introduction INTRODUCTION Acclaim Columns Dedicated to High Quality Base Asymmetry Acclaim Columns Base Performance: Amitriptyline Reliability and Durability At Dionex we know that quality and reliability are essential to a successful analysis. Our columns are thoroughly tested individually, so that chromatographers can have full confidence in them. Manufacturing starts with an ultrapure silica substrate, using only carefully selected lots with narrow ranges of physical parameters. By design, the bonding processes are clean and repeatable with no unexpected changes in performance. Each batch...

Introduction Steric Selectivity Evaluation of Metal Contamination Column: Dimensions: Mobile Phase: Temperature: Flow Rate: Comparison of Steric Selectivity for Acclaim 120 C18 and Acclaim PA Acclaim 300 C18, 3 µm 4.6 × 150 mm 50/50 v/v water/methanol 30 °C 1.0 mL/min Columns: Mobile Phase: Temperature: Flow Rate: Inj. Volume: 1. Toluene 2. Phenanthrene 3. o-Terphenyl 4. Triphenylene Column: Dimensions: Mobile Phase: Temperature: Flow Rate: Inj. Volume: Detection: As indicated 90% methanol 30 °C 1 mL/min 5 µL Metal Contamination Peaks: 1. Toluene 2. Phenanthrene 3. o–Terphenyl 4. Triphenylene...

Performance Specifications Polar selectivity ratio Base asymmetry High performance reversed-phase columns for the separation of small molecules Metal activity ratio Steric selectivity ratio Retention time Chromatographic specifications for Acclaim 120 C18 4.6 × 150 mm columns. Please refer to Part 4 for a detailed description of the tests and specifications. Specifications are subject to change at the discretion of Dionex. The Acclaim 120 C18 series columns feature a densely bonded monolayer of octadecyldimethylsiloxane on a highly pure, spherical, silica substrate with 120 Å pore structure....