Anza Restriction Enzyme Cloning System Complete, one-buffer system— for beautifully simple cloning

Cloning has never been simpler Finally, forget the frustrations of finding compatible buffers and sorting through protocols for your restriction enzyme digests—start getting reliable results for your downstream experiments. The Invitrogen™ Anza™ Restriction Enzyme Cloning System is a complete system, comprised of: 128 restriction enzymes + 5 DNA modifying enzymes Anza T4 DNA Ligase Master Mix All Anza™ restriction enzymes work together cohesively and are fully functional with the single Anza™ buffer. The system offers: • One buffer for all restriction enzymes • One digestion protocol for all...

A one-buffer system Eliminate the frustration of trying to find compatible buffers for all your enzymes. All Anza restriction enzymes allow for complete digestion using a single Anza buffer. Now you can save time and perform experiments with multiple enzymes in a single reaction without compromising your results. Triple digestion C – undigested plasmid DNA 1 – Anza 1 NotI 2 – Anza 16 HindIII 3 – Anza 15 XmaJI 4 – Anza 1 NotI + Anza 16 HindIII + Anza 15 XmaJI L – 1 Kb Plus DNA Ladder Figure 1. Anza restriction enzymes show complete digestion with three enzymes in a single buffer. Plasmid DNA (6215...

A one-protocol system Anza restriction enzymes utilize one simple two-step digestion protocol, regardless of the number of restriction enzymes in your reaction or the type of DNA you’re working with—just prepare your reaction mixture and incubate at 37°C for 15 minutes. Protocol 1. repare a reaction mix by adding reagents in the order indicated in Table 1. P 2. Incubate at 37°C for 15 minutes. As required to make up final reaction volume Nuclease-free water Anza 10X Buffer or Anza 10X Red Buffer Anza restriction enzyme 2 Anza restriction enzyme 3 Anza restriction enzyme 1 Final reaction volume...

Anza restriction enzymes consistently digest DNA completely in 15 minutes and with varying DNA substrates. L Purified PCR product Digestion of PCR DNA L – 1 Kb Plus DNA Ladder C – undigested DNA 1 – Anza 3 BcuI 2 – SpeI-HF (Competitor N isoschizomer to BcuI) 3 – Anza 9 NdeI 4 – NdeI (Competitor N) 5 – Anza 47 Eco52I 6 – EagI-HF (Competitor N isoschizomer to Eco52I) Digestion of plasmid DNA L – 1 Kb Plus DNA Ladder C – undigested plasmid DNA 1 – Anza 3 BcuI 2 – SpeI-HF (Competitor N isoschizomer to BcuI) 3 – Anza 47 Eco52I 4 – EagI-HF (Competitor N isoschizomer to Eco52I) Figure 2. Anza restriction...

A flexible system Some restriction enzymes can exhibit star activity, or a decrease in specificity to their DNA recognition site, with prolonged digestions. Star activity results in nonspecific cleavage of DNA and can occur when reaction conditions are not optimal, such as high glycerol content or presence of Mg2+. Anza restriction enzymes, in conjunction with the Anza buffer, have been optimized to allow for flexibility in digestion times—from complete digestion in 15 minutes to overnight digestions—without the worry of star activity. bp 16 hours L – 1 Kb Plus DNA Ladder C1 – undigested plasmid...

A complete system The Anza restriction enzyme cloning xystem is a complete system, inclusive of additional Anza DNA modifying enzymes that are fully functional with the Anza buffer. Now you can complete your additional cloning steps with ease. Options for DNA modification Vector 5' P Use Anza T4 PNK Kit to phosphorylate the 5' ends of PCR-amplified blunt-ended DNA. Use Anza Alkaline Phosphatase to dephosphorylate vector (digested with restriction enzyme(s) that result in compatible ends) to prevent self-ligation. Dephosphorylation of vector lowers the number of transformants without insert (background),...

Get started The Anza Starter Kits include 5 or 10 of the most common Anza restriction enzymes, Anza 10X buffer, and selected Anza DNA modifying enzymes, so you can be on your way to simpler cloning. Anza 5-pk Starter Kit Anza 1 NotI Anza 5 BamHI Anza 11 EcoRI Anza 12 XbaI Anza 16 HindIII Anza T4 DNA Ligase Master Mix Anza Alkaline Phosphatase Anza 10X Buffer Anza 10X Red Buffer Anza 10-pk Starter Kit Anza 1 NotI Anza 3 BcuI* Anza 5 BamHI Anza 8 XhoI Anza 10 DpnI Anza 11 EcoRI Anza 12 XbaI Anza 16 HindIII Anza 19 BglII Anza 26 Eco32I** Anza T4 DNA Ligase Master Mix Anza Alkaline Phosphatase Anza...

Ordering information Product Restriction enzymes Restriction enzymes

Ordering information (continued) Product Restriction enzymes Restriction enzymes

Ordering information (continued) Product DNA modifying enzymes Anza 10X Buffer Set Anza T4 DNA Ligase Master Mix Anza T4 DNA Ligase Master Mix Anza Alkaline Phosphatase Anza Alkaline Phosphatase Anza 5-pk Starter Kit Anza 1 NotI Anza T4 DNA Ligase Master Mix Anza Alkaline Phosphatase Anza 10-pk Starter Kit Anza 1 NotI Anza T4 DNA Ligase Master Mix Anza Alkaline Phosphatase

Find the right Anza enzyme for your research and order directly using the free CloningBench mobile app. Download it for iOS™ and Android™ operating systems. Find out more at thermofisher.com/anza For Research Use Only. Not for use in diagnostic procedures. © 2016 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. iOS is a trademark of Cisco Technologies. Android is a trademark of Google, Inc. COL02444 08