Chemically Defined CHO Media Test Panel Consultation Service Accelerate your time-to-market Thermo Fisher SCIENTIFIC

If you’re looking for rapid, customized solutions for media to improve titers with consultative technical support, the Gibco™ Chemically Defined (CD) CHO Media Test Panel Consultation Service is for you. Leverage our more than 50 years of experience to help you shorten your time-to-market

Contents Rapid customization 4 Technical support and consultation 6 Ordering and contact information 11

Gibco Bioproduction Services (GBS) media panel Our GBS media panel consists of 5 diverse chemically defined formulations (Table 1) produced using animal origin-free components. These formulations can be further supplemented with growth factors upon request.* The Gibco CD CHO Media Test Panel Consultation Service offers rapid access to a media library along with consultative technical support for customers wishing to test and compare multiple formulations. Our experienced technical support team can help you save time by assisting you in planning your experiment and analyzing your results. The...

These 5 formulations can be mixed to produce multiple conditions to test, depending on your resources and system capabilities (Table 2). No matter the number of conditions tested, our scientists and technical engagement team will be available at every step to assist and answer questions to help shorten your time-to-market. Table 2. Example of media mixing design strategy to produce 21 test conditions.

Your local Field Application Scientist will discuss with you how to test the panel based on your available resources and equipment: • For labs that may have limited time, personnel, lab space, and resources, your best option is to test the panel as is. • For those with additional time and space available, moderately complex media mixing with more test conditions, as shown in Table 2, is ideal. Upon completion of your experiment, your FAS and our internal R&D team will review the results and determine the best path forward. In this consultation, we will cover the following: • Review growth, titer,...

Improved titers Customizing your media for your specific cell line using our chemically defined (CD) CHO test panel media provides higher titers compared to catalog media on the market. Gibco™ CHO-S™ and DG44 cell lines, when tested using the CD CHO test panel media, were shown to have higher titers than in the control medium (catalog product) (Figure 1). Viable cell density (VCD), CHO-S cell line Viable cell density (VCD), DG44 cell line 25 Catalog medium Peak titer, CHO-S cell line Peak titer, DG44 cell line Peak titer relative to catalog medium Peak titer relative to catalog medium Control...

Upon further development, tests were performed with chemically defined CHO panel medium 4 (GBS 4) on 2 cell lines that were responsive to LONG™ R3 insulin-like growth factor-1 (IGF-1) supplementation (Repligen). We observed a 30% increase in titer in both cell lines compared to that in catalog media conditions, when IGF-1 was used (Figure 2). Peak titer, CHO-S cell line Peak titer relative to catalog medium Peak titer relative to catalog medium Catalog medium Peak titer, DG44 cell line 1.4 Peak titer relative to catalog medium Catalog medium Catalog medium Figure 2. Supplementation of chemically...

When CHO-S cells grown in CHO panel media were fed with 2X Gibco™ EfficientFeed™ C+ Supplement (2% daily, day 3–12), higher titers were observed compared to cells grown in catalog media under the same feeding conditions (Figure 3). VCD, CHO-S cell line, fed-batch 1.8 Peak titer relative to catalog medium Peak titer, CHO-S cell line, fed-batch 1.8 Peak titer relative to catalog medium Catalog medium Figure 3. Improved feed response of CHO-S cells in CD CHO panel media compared to catalog media. Our team of professionals will work with you to recommend proper feed and feeding strategies for your...

When chemically defined CHO panel medium 4 (GBS 4) was supplemented with LONG R3 IGF-1 in addition to feeding with EfficientFeed C+ Supplement, DG44 cells were positively affected (Figure 4). VCD, DG44 cell line, fed-batch 30 Peak titer, DG44 cell line, fed-batch Peak titer relative to catalog medium Catalog medium Figure 4. Supplementation of fed-batch culture of DG44 cells with LONG R3 IGF-1 increases productivity. Not all cell lines will be responsive to addition of growth factor.

Our panel ordering process keeps the end goals of rapid optimization and speed-to-market in mind. That’s why we’ve made the process short and simple with just 3 steps. To start your path to increased titers, please contact your local Account Manager or Field Application Scientist today to request a consultation, or email [email protected]

Thermo Fisher SCIENTIFIC Find out more at thermofisher.com/gibcobpdservices For Research Use Only. Not for use in diagnostic procedures. © 2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. LONG is a trademark of Repligen Corporation. COL03771 0817