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Pierce Magnetic Beads

Pierce Magnetic Beads
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Thermo Scientific Pierce Magnetic Beads magnetic bead technology for better assay development custom magnetic particles • immunoprecipitation • affinity purification

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exceptional bead technology for protein biology Thermo Scientific Pierce Magnetic Beads are available for immunoprecipitation, affinity purification as well as custom magnetic particle creation. The high-performance, iron oxide, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher Duo and KingFisher Flex Instruments. Samples can be analyzed by Western blotting or on Thermo Scientific Mass Spectrometers for quantitation of low abundant targets. 1

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Prepare cells, tissue or fluid samples with Thermo Scientific Lysis Reagents Isolate your target with one of our magnetic beads: Choose to run a few samples on the bench or create a high-throughput assay with KingFisher® Instruments • Magnetic NHS for creating custom affinity resin • Magnetic Protein A/G for immunoprecipitation • Magnetic Streptavidin for biotin pull-downs • Magnetic Glutathione for GST-tagged protein purification • Magnetic Titanium Dioxide for phosphopeptide enrichment Identify and quantitate your analyte using Thermo Scientific Mass Spectrometers and labeling reagents...

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immobilize your ligand for custom magnetic assays Magnetic Protein Immobilization Beads Thermo Scientific Pierce NHS-Activated Magnetic Beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments. The activated magnetic beads contain N-hydroxysuccinimide (NHS) functional groups that react with primary amines, forming stable amide linkages. Once they are covalently attached, the immobilized proteins are highly resistant to leaching from the bead surface. When prepared beads are used in...

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NHS-Activated Magnetic Bead Amine Ligand (Antibody) Coupled IgG (µg/mg beads) O Covalently Immobilized Ligand Figure 2. Reaction scheme for conjugation of protein onto Thermo Scientific Pierce NHS-Activated Magnetic Beads. Thermo Scientic Pierce Beads Figure 1. Significantly better coupling capacity with Thermo Scientific Pierce NHSActivated Magnetic Beads. Rabbit IgG (1mg/mL) was coupled in PBS for 2 hours at pH7.2 to 3mg each of Pierce® NHS-Activated Magnetic Beads and NHS Mag Sepharose® Beads (GE Life Sciences). Negative control beads (–) were prepared by quenching or blocking using...

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immunoprecipitation assay Magnetic Immunoprecipitation Beads The Thermo Scientific Pierce Protein A/G Magnetic Bead is a single particle that is compatible with all commonly used antibodies for immunoprecipitation (IP). These beads are coated with genetically engineered Protein A/G, a recombinant protein that combines the IgG binding domains of both Protein A and Protein G. This combination enables the capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and...

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Rabbit Polyclonal Thermo Scientic Pierce Protein A/G Dynabeads Protein G Dynabeads Protein A Rabbit Polyclonal Figure 5. The rabbit IgG binding capacity of Thermo Scientific Pierce Protein A/G Magnetic Beads is approximately four times greater than that of the leading brand of Protein A and Protein G beads. Pierce Protein A/G Magnetic Beads or Dynabeads Protein A or Protein G (Life Technologies) were added to a 96 deep-well plate (1mg beads per well). Using the KingFisher 96 Instrument, the beads were incubated for one hour with varying amounts of purified rabbit IgG (20 to 200µg). After...

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Magnetic Immunoprecipitation (IP) Kits Thermo Scientific Pierce Magnetic IP/Co-IP Kits are optimized to isolate protein complexes from biological samples. Each kit contains all the required buffers and beads validated to deliver the best results. Three versions of the kit are available to perform a classic IP, crosslink IP or direct IP. Kit Highlights • Compatible with any antibody • Faster IPs for less background • Easily capture transient protein complexes • No antibody contamination in your eluted sample • Simple handling with no sample loss • Validated for automated protocols using...

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The Pierce Crosslink Magnetic IP/Co-IP Kit uses crosslinkers The Pierce Direct Magnetic IP/Co-IP Kit uses Pierce NHS-Activated to immobilize your primary antibody to Protein A/G. This prevents antibody contamination in your eluted sample and eliminates antibody interference in Western blot and mass spec applications. Magnetic Beads to immobilize your primary antibody directly to the bead surface. This method is independent of antibody species and prevents antibody contamination in your eluted sample. Select this version: Select this version: • To eliminate antibody contamination that...

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perform high-capacity protein purification Magnetic Biotin Pull-Down Life Technologies Dynabeads MyOne T1 Beads • Stable immobilization chemistry – streptavidin is immobilized using leachresistant chemistry • High capacity – superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples • Low nonspecific binding – stable, pre-blocked beads provide clean purification products that are compatible with mass spectrometry analysis • Superior performance – nearly three times higher binding capacity than typical beads from other...

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Magnetic GST-Protein Purification Thermo Scientific Pierce Glutathione Magnetic Beads provide a simple, rapid and reliable method for the purification of glutathione S-transferase (GST) fusion proteins from crude cell lysate prepared from bacteria, yeast or mammalian cells. These beads can be used to isolate GST-tagged proteins or perform pull-down assays using GST-tagged proteins as bait. Replicate Purication Wells Highlights • High binding – 5-10mg GST/mL settled beads • Stable-affinity ligand – glutathione is covalently immobilized to particles, ensuring leach-resistance and clean...

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Pierce Magnetic Beads 16Pages

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Pierce Magnetic Beads
16Pages