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Platinum DNA polymerases

Platinum DNA polymerases
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Platinum DNA polymerases A hot start towards your PCR destinations—designed for exceptional specificity, fidelity, and versatility

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Unique features of new Platinum PCR enzymes Hot-start technology Hot-start PCR offers a greater yield of the target amplicon, with the added convenience of room-temperature reaction setup. Invitrogen™ Platinum™ DNA polymerases utilize Platinum hot-start technology, which is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing nonspecific amplification and primer degradation. Universal primer annealing A universal annealing temperature of 60°C for primers helps reduce PCR optimization steps and helps save time when working with...

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No need to calculate primer annealing temperature High success with amplification of 20–40 kb sequences Human gDNA Annealing temperature calculated for primers (°C) 59 Figure 2. PCR amplification with high specificity and yield using a universal annealing temperature of 60°C. Primer sets with various optimal annealing temperatures were used to amplify 12 targets in human genomic DNA (gDNA) with a 60°C annealing temperature. Figure 4. Amplification of long fragments. A 20 kb target from human gDNA and 30–40 kb targets from E. coli gDNA were successfully amplified using Platinum SuperFi II...

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Platinum II Taq Hot-Start DNA Polymerase Engineered enzyme for high specificity and yield with fast cycling Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase is engineered for speed, specificity, and inhibitor tolerance. Its universal primer annealing feature reduces optimization steps and allows co-cycling of different PCR assays. 250 ng Highlights • 4x faster DNA synthesis than standard Taq DNA polymerase High sensitivity and specificity • Improved specificity, sensitivity, and yields • High tolerance to common PCR inhibitors • Efficient amplification of AT-rich and GC-rich targets...

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Platinum Direct PCR Universal Master Mix Universal master mix for a direct route to PCR Invitrogen™ Platinum™ Direct PCR Universal Master Mix is designed to amplify DNA sequences directly from a variety of sample types, without the need for DNA purification. The master mix contains a green dye for direct loading of PCR products onto agarose gels. Direct protocol Highlights • Direct DNA amplification from a variety of sample types • PCR protocols with the universal annealing temperature Direct • Flexible workflow, fast time (<1 hour)+ primers • Multiplexing with up to 5 targets Two protocols...

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Resources • Application notes at thermofisher.com/pcr-appnotes • Technical and how-to videos at thermofisher.com/pcr-videos • Education and technical tips at thermofisher.com/pcr-education • PCR workflow brochure at thermofisher.com/pcr-brochure • Molecular biology handbook at thermofisher.com/molbiobook PCR workflow solutions Explore a fleet of Applied Biosystems™ and Invitrogen™ PCR products to reach your scientific destinations with direct routes and first-class results. Upgrade your PCR at thermofisher.com/amplifly Reverse transcriptases Oligo design Thermal cyclers Ordering information...

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