Platinum DNA polymerases A hot start towards your PCR destinations—designed for exceptional specificity, fidelity, and versatility

Unique features of new Platinum PCR enzymes Hot-start technology Hot-start PCR offers a greater yield of the target amplicon, with the added convenience of room-temperature reaction setup. Invitrogen™ Platinum™ DNA polymerases utilize Platinum hot-start technology, which is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing nonspecific amplification and primer degradation. Universal primer annealing A universal annealing temperature of 60°C for primers helps reduce PCR optimization steps and helps save time when working with multiple...

No need to calculate primer annealing temperature High success with amplification of 20–40 kb sequences Human gDNA Annealing temperature calculated for primers (°C) 59 Figure 2. PCR amplification with high specificity and yield using a universal annealing temperature of 60°C. Primer sets with various optimal annealing temperatures were used to amplify 12 targets in human genomic DNA (gDNA) with a 60°C annealing temperature. Figure 4. Amplification of long fragments. A 20 kb target from human gDNA and 30–40 kb targets from E. coli gDNA were successfully amplified using Platinum SuperFi II DNA...

Platinum II Taq Hot-Start DNA Polymerase Engineered enzyme for high specificity and yield with fast cycling Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase is engineered for speed, specificity, and inhibitor tolerance. Its universal primer annealing feature reduces optimization steps and allows co-cycling of different PCR assays. 250 ng Highlights • 4x faster DNA synthesis than standard Taq DNA polymerase High sensitivity and specificity • Improved specificity, sensitivity, and yields • High tolerance to common PCR inhibitors • Efficient amplification of AT-rich and GC-rich targets Fast...

Platinum Direct PCR Universal Master Mix Universal master mix for a direct route to PCR Invitrogen™ Platinum™ Direct PCR Universal Master Mix is designed to amplify DNA sequences directly from a variety of sample types, without the need for DNA purification. The master mix contains a green dye for direct loading of PCR products onto agarose gels. Direct protocol Highlights • Direct DNA amplification from a variety of sample types • PCR protocols with the universal annealing temperature Direct • Flexible workflow, fast time (<1 hour)+ primers • Multiplexing with up to 5 targets Two protocols to...

Resources • Application notes at thermofisher.com/pcr-appnotes • Technical and how-to videos at thermofisher.com/pcr-videos • Education and technical tips at thermofisher.com/pcr-education • PCR workflow brochure at thermofisher.com/pcr-brochure • Molecular biology handbook at thermofisher.com/molbiobook PCR workflow solutions Explore a fleet of Applied Biosystems™ and Invitrogen™ PCR products to reach your scientific destinations with direct routes and first-class results. Upgrade your PCR at thermofisher.com/amplifly Reverse transcriptases Oligo design Thermal cyclers Ordering information Product...