The complete BioPharma characterization solution

“BioPharma Option” for the Thermo Scientific™ Q Exactive™ Plus and Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometers adds superior denatured and native MS intact analysis and subunit top/ middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available. When combined with Thermo Scientific™ BioPharma Finder™ software it provides a complete integrated hardware and software solution for BioPharma characterization. The Thermo Scientific™ Q Exactive™ BioPharma platform offers distinct operational modes that have been optimized for the top 3 protein...

High Muss Range |KMR) Mode Protein Mode Standard Mode Peptide Mapping V «** Optimized intact protoin analysis under both native and denaturing conditions assures the highest quality and most informative spectra for the widest range of therapeutic proteins. Extreme resolving power of (he Orbitrap mass analyzer ensu res isotopic resolution of subunits and facilitates top/middie-down sequencing. Perform peptide mapping with Qrbitrap technology for unparalieled acquisition speed, mass accuracy, and spectral quality.

Robust mass accuracy and efficient desolvation have become hallmarks of Qrbitrap technology for intact protein analysis. Based on the engineering behind the t hermo Scientific" Exactive Plus' EMR Orbitrap" mass spectrometer we have developed High Mass Range (HMR) mode ter intact protein analysis on the BicF^hanma- ready Q Exactive Plus and Q Exactive HF mass spectrometer. HMR mode was designed specifically tor intact therapeutic protein workflows with Ihe aim of provi: :ing ease of use and flexibility as needed. We have improved transmission and scanning up to m/z 3000 resulting in increased...

St ate-of'the-art intact protein analysis The introduction of I-IMR mode brings the highest quality and sensilivity for intact protein analysis to the Q Exactive series instruments. The 500 pg on-column injection demonstrates the superior sensitivity and mass accuracy achieved with the Q Exactive Plus running in the new High Mass Range mode.

High Mass Range (HMR) mode enables increased scanning range up lo 8.000 m/z. providing the flexibility lo analyze you1 intact proteins in either denaturing or native MS conditions. Native MS is best for co-eluting high complexity samples like ADCs. Conventional denaturing conditions, including mobile phase containing organic and acidic pH, result in protein unfolding and increased protonation. Denaturing MS is compatible with reverse phase chromatography, which allows sensitive, salt-tree conditions tor identifying intact proteins at lower fft/Z ranges. Native MS conditions using volatile sail-based...

Multiple Analysis Options. HMR mode provider accurate intact mAb analysis under both denaturing and native MS conditions allowing the flexibility to analyze a diverse range of therapeutics under optimized conditions.

with Size Exclusion Chromatography Or Native MS is now as easy as LC-MS lmad protein analysis under native MS conditions can now be performed i outineiy using size exclusion chromatography (SEC) coupled to the 0 Exactive Plus or O Exactive HF mass spectrometers. The Thermo ScfentHkT Vanquish UHFLC system cm nbitiad with the Thermo Scientific" MAbPac' SEC-1 Size Exclusion Cciumn allows automatic butter exchange 1o be performed in-line with the mass spectrometer. High throughput native LC-.MS intact protein am-! -. 3 g with the MAbPac SEC-1 column enables greater-than-baseline separation of antibody...

m.Measuring DAR usinghigh resolution native MS. I The next generation ot high resolution native MS has an Performing intact protein analysis in native MS allows greater • * separation between seiguential / charge states. For ADS SartTfSfe^ native MS allows the low and ' high abundance DAR fb?ftis to be separated and thus accurately measured. Native MS DAR analysis ot complex ADC is now clear enough \q *ti by eye. . r Vanquish UHPLC Sliding Window Re SpecT Deconvolution for highly accurate average masses

Subunit and middle-down analysis with Protein modeQ Exactive Plus with BioPharma Finder software Baseline isotopic resolution o' mAh subunits is routinely achievable in Protein mode. The cysteine protease produced by S. pyogenes, known as IdeS, is highly specific for IgG antibody rnolecules. IdeS digestion followed by reduction will generate 3 subunits which are readily separated by reverse phase HPLC using the thermo Scientific” MAbPac” RP. Deconvolution pf subunit MS spectra is easy and powerful with Thermo Scientific" BioPharma Finder” software. The Sliding Window method and the Thermo Soon...

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Reduced antibody and top-down analysis with Protein modeQ Exactive HF with BioPharma Finder software Protein mode on trie Q Exactive HP is a powemrl combination that has sot the standard for the highest quality isotopically resolved data achievable on a benchtop mass spectrometer. the MAbRac HP provides complete separation of reduced mAb light end heavy chains. Full MS scans using high (120.000) and low : 15.OOO) resolution allow mass determination of the light and heavy chains, respectively. The ultra-high'-field Thermo Scientitle' D2G Orbitrap’ mass analyzer allows baseline isotopic resolution...