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QuantStudio™ 3

QuantStudio™ 3
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QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems Just the right everything

Data within your reach Applied Biosystems™ QuantStudio™ 3 and 5 Real-Time PCR Systems allow you to remotely monitor your runs, as well as easily access and securely share results with colleagues anywhere, anytime with Connect, our cloud-based platform. When your data are always within reach, the answers shaping the future of science are never far away. Interactivity • Run and edit directly from touchscreen Accessibility* • Access experiment runs from any location, anytime, with remote monitoring • Wi-Fi–enabled connectivity • Utilize portable devices to quickly analyze data when you need to...

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Simple, intuitive interface—at your fingertips The interactive touchscreen interface and simplified Applied Biosystems™ QuantStudio™ Design and Analysis Software make it easy to get started and stay organized. • Manipulate view to a particular graph or data point (Figure 2B) • Software can be accessed either via desktop or online • Preoptimized protocol templates allow quick selection of default protocols for standard applications • Easily identifiable icons guide you through the workflow to set up runs and analyze experiments • Option to pause a real-time PCR run on demand • Locked...

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Technical specifications QuantStudio 3 Real-Time PCR System QuantStudio 5 Real-Time PCR System Sample capacity (wells) Reaction volume 96-well 0.1 mL block: 10–30 μL 96-well 0.2 mL block: 10–100 μL 384-well block: 5–20 μL Bright white LED Excitation source Optical detection 96-well: 6 decoupled filters 384-well: 5 coupled filters Excitation/detection range Maximum block ramp rate Average sample ramp rate Temperature uniformity Temperature accuracy Compatible dyes FAM™/SYBR™ Green, VIC™/JOE™/HEX™/ TET™, ABY™/NED™/TAMRA™/Cy®3, JUN™, ROX™/Texas Red™ FAM/SYBR Green, VIC/JOE/HEX/TET,...

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Flexible, powerful secondary analysis software Applied Biosystems™ Analysis Modules are innovative cloud-based data analysis applications that bring together multiple data sets in one convenient place and render them in stunning data visualizations for enhanced analysis and insights (Figures 3–5). Figure 3. Absolute quantification module for gene expression analysis. The module enables analysis of genes of interest with the use of a standard curve. Additional flexibility is achieved by importing standard curves from other experiments. Figure 4. Relative quantification module for gene...

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High-quality data for a variety of applications Utilizing proven Applied Biosystems™ OptiFlex™ technology and VeriFlex™ Blocks, QuantStudio 3 and 5 systems offer optimal data accuracy and sensitivity (Figure 6). Applications include analysis of gene expression, microRNAs and noncoding RNAs, SNPs, copy number variation, somatic mutations, drug metabolism enzymes, and protein expression. Figure 6. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid...

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Assay flexibility to support your application The QuantStudio 3 and 5 systems support probe-based assays as well as intercalating dyes (Figures 7–9). TaqMan probe-based assays, developed with powerful algorithms and optimized master mixes, enable outstanding specificity and sensitivity. Applied Biosystems™ SYBR™ Green chemistry is an economical alternative for target identification or initial qualification of assays. Figure 7. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems™ SYBR™...

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Instrument with service—Cat. No.* QuantStudio 3 Real-Time PCR System (96-well, 0.1 mL block)** QuantStudio 3 Real-Time PCR System (96-well, 0.2 mL block)** QuantStudio 5 Real-Time PCR System (96-well, 0.1 mL block)** QuantStudio 5 Real-Time PCR System (96-well, 0.2 mL block)** QuantStudio 5 Real-Time PCR System (384-well block)** Instruments QuantStudio 3 system configurations QuantStudio 5 system configurations * Extended warranty packages for the QuantStudio 3 and 5 Real-Time PCR Systems include the instrument, SmartStart™ Orientation, and a 1-year AB Assurance service plan with 1 planned...

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QuantStudio™ 3
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