Thermo Scientific Reagents, Solvents and Accessories Part of Thermo Fisher Scientific Gas Chromatography High Performance Liquid Chromatography Mass Spectrometry

Thermo Scientific Products for GC and HPLC Derivatization Reagents for Specific Functional Groups 1 Derivatization Reagents for Drug-of-Abuse 2-3 Specialized Products and Reagents 20 HPLC Ion Pair Reagents 24-40 Binding and Elution Buffers for Affinity Purification 25 HPLC Ion Pair Reagents 26 HPLC/Spectrophotometric Grade Solvents 27 Derivatization and Visualization Reagents for HPLC 28-30 Amino Acid Analysis 32-41 Sample Preparation and Hydrolysis 34 Fluorometric Detection Reagents for Amino Acids 36 Ninhydrin Detection Reagents for Amino Acids 37-38 High-Purity Pre-Column Derivatization Reagents...

Derivatization Reagents for Specific Functional Groups Functional Group Silylation BSA BSTFA BSTFA+TMCS Amides MSTFA O MSTFA+TMCS O Tri-Sil Reagents –C–NH2 MTBSTFA –C–NH2 Primary MTBSTFA+TBDMCS O Acylation MBTFA O TFAA –C–NHR –C–NHR PFAA TMS Amides Difficult to form due to steric hindrance TMS Amides TMS Amides TMCS used as a catalyst TMS Amides Reaction byproducts more volatile TMS Amides TMS Amides TBDMCS Amides Difficult to form; very stable TBDMCS Amides TBDMCS aids derivatization Trifluoroacetamides Trifluoroacetamides Pentafluoropropionamides Secondary HFBA Alkylation MethElute Reagent...

Derivatization Reagents for Drugs-of-Abuse Reagent Reference Amphetamines Amphetamines BSTFA 1 NH2 Amphetamines HFAA 2-5 CH Amphetamines HFAA/PFAA 6 C6H5 CH2 CH 3 Amphetamines MSTFA with TMCS 7 Amphetamines TFAA 7,8 Methamphetamine TFAA 9,10 Barbiturates BSTFA 1 MethElute Reagent (TMPAH) 11-13 O O NH Methylate Reagent (DMFDMA) 14,15 CH2 CH CH2 PFBBr 16 CH3 Cocaine Benzoylecgonine CH3 N COOH BSTFA/Butyl Iodine/TMAH 17 BSTFA 1,18 MTBSTFA 19 PFAA/PFPOH 9,20 BSA 21 BSTFA 22 MSTFA 21 TFAI 23 Marijuana THC metabolites COOH OH BSA 24 BSTFA/BSTFA+1% TMCS 24-27 BSTFA/TMCS/TMSI 24 MSTFA 9 MSTFA/MSTFA+1%...

References   1. Dutt, M.C. (1982). J. Chromatogr. 248, 115-124.   2. Wu, A.H.B., et al. (1992). J. Anal. Toxicol. 16, 137-141.   3. Thurman, E.M., et al. (1992). J. Anal. Toxicol. 16, 19-27.   4. Reimer, M.L., et al. (1993). Biol. Mass Spectrom. 22, 235-242.   5. Yamamoto, T., et al. (1989). J. Anal. Toxicol. 13, 117-119.   6. Wu, A.H.B., et al. (1992). Biol. Mass Spectrom. 21, 278-284.   7. ood, H.D. and Knitter, J.A. (1991). Capillary Chromatography. R W.G. Jennings, ed., pp. 115-130.   8. DePace, A., et al. (1990). J. Forensic Sci. 35(6), 1431-1435.   9. Mulé, S.J. and Casella, G.A. (1998)....

Introduction to Gas Chromatography Reagents Efficiency Direct analysis can be difficult when compounds interact with each other or with the column. These interactions can lead to poor peak resolution and/or asymmetrical peaks that make proper peak integration difficult or impractical. This interference can be reduced with conversion to derivatized products.13,14 Compounds that exhibit co-elution can often be separated by using the appropriate derivatization methods. Detectability As demand increases for the analysis of increasingly smaller amounts of materials, it becomes important to extend...

Thermo Scientific Silylation Reagents Silylation and Silylation Reagents Only Thermo Scientific Reagents offer the combination of variety, quality and reliability. Silyl derivatives are the most widely used derivatives for gas chromatographic applications. Usually they are formed by the replacement of the active hydrogens from acids, alcohols, thiols, amines, amides and enolizable ketones and aldehydes with the trimethylsilyl group. A variety of reagents is available for the introduction of the trimethylsilyl group. These reagents differ in their reactivity, selectivity and side reactions and...

Thermo Scientific Silylation Reagents For excellent chromatographic separations. The reagent to choose for difficult-to-silylate compounds. CH 3 BSTFA is a powerful trimethylsilyl donor, with donor strength that is comparable to its unfluorinated analog BSA [N,O-Bis(trimethylsilyl) acetamide]. BSTFA reacts to replace labile hydrogens on a wide range of polar compounds with a -Si(CH3)3 group. This physical characteristic is particularly useful in the gas chromatography of some lower boiling TMS-amino acids and TMS Krebs cycle acids. PROTOCOL Thermo Scientific BSTFA + 1% TMCS is ideal for derivatizing...

MSTFA and MSTFA 1% TMCS Offers maximum volatility. The perfect reagent for volatile TMS derivatives. CH 3 Highlights: • Trimethylsilyl donor strength comparable to BSA and BSTFA • Reacts to replace labile hydrogens on a wide range of polar compounds with a -Si(CH3)3 group • Used to prepare volatile and thermally stable derivatives for GC and MS • Primary advantage of Thermo Scientific MSTFA is the volatility of its byproduct, N-methyltrifluoroacetamide; MSTFA is the most volatile TMS-amide available which has an even lower retention time than MSTFA • Often TMS derivatives of small molecules can...

Thermo Scientific Silylation Reagents MTBSTFA and MTBSTFA + 1% TBDMCS Offers stable TBDMS (tert-butyldimethylsilyl) derivatization. The strongest hydroxyl silylator available for carbohydrates and steroids. Sakauchi and Horning have shown TMSI to be an all-purpose reagent for unhindered steroids to highly hindered steroids. Highlights: • Derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines • Typical yields are >96% • Provides TBDMS ethers that are 104 times more stable to hydrolysis than TMS ethers • Reaction byproducts are neutral and volatile • Derivatives have a high molecular...

The popular choice for silylation of sugars and related substances. An excellent catalyst for difficult-to-silylate compounds. Thermo Scientific HMDS greatly extends the practical range of GC, improving chromatographic results in the silylation of sugars A critical study of the optimal proportions of HMDS and trimethyl- chlorosilane for producing maximum yield of trimethylsilyl derivatives was conducted by Sweeley, etal. This protocol describes the method of Sweeley, etal. for the trimethylsilylation of sugars and related substances. 1. Combine 10 mg or less carbohydrate sample, 1.0 ml anhydrous...