Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer Unmatched analytical performance

Transforming biological research In the world of systems and structural biology, research objectives continue to become more challenging: digging deeper into the proteome, identifying lower abundance analytes in more-complex samples, making quantitative comparisons over more experimental conditions, elucidating structures faster. Meeting such challenges is the goal of the Thermo Scientific™ Orbitrap Fusion™ mass spectrometer. Its revolutionary Thermo Scientific™ Tribrid™ architecture combines the best of quadrupole, Orbitrap, and linear ion trap mass analyzers in a new class of instrument. The...

Identify more analytes more quickly Increased sensitivity, scan rate, and mass resolution enhance the ability to positively identify more low-abundance proteins, such as transcription factors, in less time. Productivity is enhanced through the massive parallelization enabled by Tribrid architecture and Dynamic Scan Management. Quantify more accurately Synchronous Precursor Selection combined with MS3 significantly improves quantitative accuracy when using isobaric mass tags for relative quantitation of proteins. Elucidate structures more thoroughly The ability to use any fragmentation mode, at...

Revolutionary Tribrid architecture Tribrid architecture, with an independent ion-routing multipole controlled by Dynamic Scan Management, enables massive parallelization, significantly increases scan rates, and maximizes versatility. Any of three dissociation techniques—CID, HCD, and ETD—can be performed at any fragmentation stage, followed by analysis in either the linear ion trap or Orbitrap mass analyzer. Excellent sensitivity and selectivity Precursor selection using a quadrupole mass filter allows the ion trap and Orbitrap mass analyzers to operate in parallel. Wide mass range isolation...

Unsurpassed resolution and increased scan rate Ultra-high-field Orbitrap mass analyzer offers resolution in excess of 500,000 for unsurpassed separation of isobaric interferences. This novel Orbitrap design also allows for MS/MS scan rates up to 20 Hz, with unmatched spectral quality. Longer detector life Large-surface-area detector has two dynodes to capture the complete ion flux from the ion trap. The single multiplier features a very large surface area for extended lifetime. Multistage precursor ion activation (MS n) and sensitive mass analysis Dual-pressure configuration of the linear ion...

The Universal Method eliminates method optimization for data-dependent analysis In order to maximize the number of unique peptides identified, injection times is one parameter to optimize with low and high peptide loads. The Universal Method, with no optimization, is shown here to perform equally as well as manual optimization without the added time and sample. The Universal Method: A Single Method Optimal for Multiple Sample Types 6000 Therapeutic proteins and monoclonal antibodies have transformed biotechnology and the pharmaceutical industry. Essential to the development of new biotherapeutics...

More accurate protein quantification Multiplexed analysis using isobaric mass tags such as TMT™ reagents or iTRAQ® reagents is a powerful tool, enabling quantitative comparison of protein abundances across time, conditions, tissues, subcellular locations, or other experimental variables. In conventional mass tagging experiments, reporter ions are generated at the MS2 stage. However, in complex samples, co-isolation of isobaric background peptides distorts reporter ion ratios, yielding inaccurate quantitation and masking subtle but biologically significant changes in abundance. • Goal: • Approach:...

Simple, reliable ETD enhances characterization of complex PTMs Electron-transfer dissociation (ETD) is a powerful complement to CID and HCD for the analysis of proteins. It cleaves primarily along the peptide backbone, generating c and z ions, often with increased fragment coverage compared to b and y ions that dominate CID spectra. ETD leaves side chains and modifications largely intact, making it particularly useful when analyzing post-translationally modified proteins. The optional Thermo Scientific™ EASY-ETD™ ion source, designed specifically for the Orbitrap Fusion MS, is robust, easy to...

Multiple fragmentation types provide exceptional sequence coverage for top-down analyses The Orbitrap Fusion MS has the high resolution and accurate mass necessary to fully resolve monoclonal antibody heavy and light chains, as well as to accurately assign the complex fragmentation spectra generated from top-down experiments on large species. To maximize fragmentation for sequence characterization, proteins can be analyzed using a combination of fragmentation types including CID, HCD, ETD, and EThcD which provide complementary information. More complex experiments such as MSn analyses using any...

Determine structures more quickly and accurately Resolution of isobaric interferences Relative Abundance With mass resolution in excess of 500,000, the Orbitrap Fusion mass spectrometer can easily separate and identify isobaric compounds indistinguishable by other MS technologies. Structural characterization on a UHPLC timescale Relative Abundance The Tribrid architecture and Dynamic Scan Management of the Orbitrap Fusion MS deliver exceptional scan rates for applications where high throughput and fast chromatography demand the most MSn data from a single run. The quadrupole mass filter, ion-routing...

Maximum performance with less effort The Orbitrap Fusion Tribrid mass spectrometer delivers more, higher-quality information from more types of samples faster than any mass spectrometer available today. But more is only part of the story; the other part is less - less effort. The intelligence built into the Orbitrap Fusion instrument and software makes it possible to achieve exemplary results with far less effort than required by previous generations of mass spectrometers. This built-in intelligence helps researchers focus on their science instead of method development and instrument operation....