Thermo Scientific Varioskan™ LUX User Manual SkanIt™ Software for Microplate Readers

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P Preface About This Guide Thermo Scientific™ Varioskan™ LUX is a modular multi-technology microplate reader and it is controlled by Thermo Scientific SkanIt® Software for Microplate readers. This guide gives an overview of the instrument and software installation procedures, and describes the main operations and routine maintenance. Related Documentation In addition to this guide, Thermo Fisher Scientific provides the following electronic documents for Varioskan LUX and SkanIt Software: • Thermo Scientific™ Varioskan™ LUX Technical Manual (Cat. No. N16045) • Thermo Scientific™ SkanIt™ Software...

Contacting Us For the latest information on products and services, visit our website at: www.thermofisher.com/platereaders Thermo Scientific Varioskan™ LUX User Manual Thermo Fisher Scientific

C Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i About This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i Safety and Special Notices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i Contacting Us. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

Main Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 Application Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 Session Tree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Task Ribbon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Select the Software Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 Chapter 5 Using SkanIt Software. . ....

1 Introduction to Varioskan LUX Thermo Scientific™ Varioskan™ LUX is a modular multi-technology microplate reader. Varioskan LUX is controlled by Thermo Scientific™ SkanIt™ Software for Microplate Readers. End point, kinetic, spectral and multipoint measurements can be carried out in the UV/Vis/NIR range from appropriate microplate formats. In fluorescence intensity, time-resolved fluorescence, luminescence and AlphaScreen™ measurements 6- to 1536-well plates can be used, and correspondingly 6- to 384-well plates in absorbance measurements. Figure 1. Thermo Scientific™ Varioskan™ LUX The instrument...

Introduction to Varioskan LUX Intended Use • Monochromators are used in absorbance and fluorescence intensity measurements. • Filters are used in AlphaScreen and TRF measurements. • Most luminescence measurements do not require any wavelength selection. But if required, filters can be used. The LAT module has built-in excitation filters for TRF and AlphaScreen. The TRF and AlphaScreen (and luminescence) emission filters you need to install yourself. Note All measurement technologies, except AlphaScreen, allow spectral scanning measurements with monochromators. The instrument has an incubator...

Introduction to Varioskan LUX Instrument Layout Instrument Layout The front view of the instrument. Figure 2. The power switch (1), LED indicator (2), dispenser sliding cover (3), front cover (4), and measurement chamber door (5). The back view of the instrument. Figure 3. Thermo Fisher Scientific The cooling fan outlets (1), USB connector (2), mains power supply connector (3), power switch (4), and gas connectors (5). Thermo Scientific Varioskan™ LUX User Manual

Introduction to Varioskan LUX Instrument Layout The instrument with the dispenser sliding cover open. Figure 4. Plate In/Out button (1), LAT module (2), Prime and Empty buttons (3), dispensers 1 and 2 (4), reagent bottle holder (5). Thermo Scientific Varioskan™ LUX User Manual Thermo Fisher Scientific

2 Install Varioskan LUX This chapter describes how to install the instrument. IMPORTANT The instrument weighs 53 - 60 kg (117 - 131 lbs.) and needs two persons to lift. Take into account the weight, safety and instrument requirements when deciding where to place the instrument. Installation For detailed environmental requirements, see the Thermo Scientific™ Varioskan™ LUX Technical Manual. IMPORTANT Do not touch or loosen any screws or parts other than those specified in the instructions. Place the instrument on a sturdy laboratory bench that can take the weight of the instrument. Installation...

Install Varioskan LUX Installation Dispenser sliding cover (1). b. Remove the front panel by pulling the two bolts, one on each side at the bottom, sideways, then by turning the bottom of the cover 30°, and lifting it off. Figure 6. Side bolts (1), front panel (2). The metallic transport lock support is fastened with four screws. Two screws (1 and 2) are fastened to the tray holder, and two screws (3 and 4) are fastened to the track mechanism bar. c. Remove the tray holder screws (1 and 2), and the transport lock tag. Store the lock tag for future use. Thermo Scientific Varioskan™ LUX User Manual...