INNATE IMMUNITY AND REGENERATION The immune system plays a critical role in tissue development, homeostasis and repair. Monocytes and macrophages are the first to respond to tissue damage and are required to successfully start the regeneration process. Through the release of chemokines, cytokines, matrix remodeling proteins and growth factors, they interact with the "niche of damage", i.e. the microenvironment where tissue damage occurred. They can differentiate into osteoclasts and promote bone remodeling; they also produce the BMP-2 growth factor, favoring the osteogenic fate of the mesenchymal...

MONOCYTES device

1 - WITHDRAWAL Open the kit on the sterile field and assemble it as illustrated. Load the withdrawal syringes with 10% of anticoagulant (ACD-A); e.g. 6 ml for 60 ml of blood. Recommended withdrawal volume is 60-120 ml. Cleanse the puncture area and aspirate the needed peripheral blood volume with the provided needle. A 2 – PROCESSING Hang the kit on a stand, holding the upper bag (A) up. Insert the withdrawn blood through the upper connector (a) to fill the upper bag (A) and open the clamps (1 and 2) to activate the circuit. Wait until all the blood has passed through the filter. Put 10 ml of saline...

ADVANTAGES REGENERATIVE POTENTIAL: о Selective recovery of white blood cells from peripheral blood with red blood cell depletion о Enrichment of mononuclear cells from peripheral blood with regenerative potential о Significant reduction of granulocytes with pro-inflammatory activity QUICK PROCEDURE: о Centrifuge-independent procedure that limits cell stress о Closed-loop system with minimization of contamination risks о Fast, reproducible, and versatile procedure in different clinical indications After Monocytes processing, the final blood sample is enriched in mononuclear cells (monocytes and lymphocytes)...

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