Monocytes

Product catalog summary

Overview: The document outlines the technology and procedures developed by Tiss'You, a regenerative company, focusing on the use of monocytes for tissue regeneration. The technology involves a size-dependent selection of blood cells, particularly mononuclear cells, which are crucial for tissue repair and regeneration.

Dimensional Filtration Technology: This technology leverages the immune system's role in tissue development and repair. Monocytes and macrophages are highlighted as key players in responding to tissue damage by releasing chemokines, cytokines, and growth factors, which aid in the regeneration process.

Innate Immunity and Regeneration: Monocytes can differentiate into osteoclasts for bone remodeling, regulate angiogenesis and arteriogenesis, recycle oxidized elements in synovial fluid, and release IGF-1 to stimulate muscle stem cells and repair damaged matrices.

Monocytes Device Procedure: The procedure involves three main steps:

Withdrawal: Blood is drawn using a kit with anticoagulant, with a recommended volume of 60-120 ml.
Processing: The blood is processed through a filter to trap cells, followed by a saline wash.
Recovery: The trapped cells are recovered through gentle backwashing with saline.

Advantages: The procedure allows for selective recovery of white blood cells, enrichment of mononuclear cells, and reduction of pro-inflammatory granulocytes. It is centrifuge-independent, minimizing cell stress and contamination risks, and is applicable in various clinical settings.

Indications: The enriched blood sample can be used in orthopaedics for infiltration therapy or scaffold enrichment, in wound healing with advanced dressing, and in vascular surgery for infiltration therapy.

Contact Information: Tiss’You Srl, Strada di Paderna 2, 47895 Domagnano (RSM), Phone: +39 0549 96 45 37, Email: [email protected]

Catalog excerpts

INNATE IMMUNITY AND REGENERATION The immune system plays a critical role in tissue development, homeostasis and repair. Monocytes and macrophages are the ﬁrst to respond to tissue damage and are required to successfully start the regeneration process. Through the release of chemokines, cytokines, matrix remodeling proteins and growth factors, they interact with the "niche of damage", i.e. the microenvironment where tissue damage occurred. They can diﬀerentiate into osteoclasts and promote bone remodeling; they also produce the BMP-2 growth factor, favoring the osteogenic fate of the mesenchymal...

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MONOCYTES device

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1 - WITHDRAWAL Open the kit on the sterile ﬁeld and assemble it as illustrated. Load the withdrawal syringes with 10% of anticoagulant (ACD-A); e.g. 6 ml for 60 ml of blood. Recommended withdrawal volume is 60-120 ml. Cleanse the puncture area and aspirate the needed peripheral blood volume with the provided needle. A 2 – PROCESSING Hang the kit on a stand, holding the upper bag (A) up. Insert the withdrawn blood through the upper connector (a) to ﬁll the upper bag (A) and open the clamps (1 and 2) to activate the circuit. Wait until all the blood has passed through the ﬁlter. Put 10 ml of saline...

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ADVANTAGES REGENERATIVE POTENTIAL: о Selective recovery of white blood cells from peripheral blood with red blood cell depletion о Enrichment of mononuclear cells from peripheral blood with regenerative potential о Signiﬁcant reduction of granulocytes with pro-inﬂammatory activity QUICK PROCEDURE: о Centrifuge-independent procedure that limits cell stress о Closed-loop system with minimization of contamination risks о Fast, reproducible, and versatile procedure in diﬀerent clinical indications After Monocytes processing, the ﬁnal blood sample is enriched in mononuclear cells (monocytes and lymphocytes)...

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Biography Tiss’You Srl Strada di Paderna 2 47895 Domagnano (RSM) www.tissyou.com Phone Email

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