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Cell biology Related Antibody

Cell biology Related Antibody
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Cell biology Related Antibody

Product catalog summary
Mycoplasma Detection
  • TransDetect® Luciferase Mycoplasma Detection Kit: Utilizes a unique enzyme for bioluminescence-based detection, ensuring high sensitivity and accuracy.
  • TransDetect® PCR Mycoplasma Detection Kit: Uses PCR to detect mycoplasma DNA with high specificity, capable of detecting as few as 20 genome copies.
  • TransSafeTM Mycoplasma Elimination Reagents: Includes TransMyco-1+2 and TransMyco-3, which effectively eliminate mycoplasma with low cytotoxicity using antibiotics.
  • TransSafeTM Mycoplasma Prevention Reagent: Prevents mycoplasma and bacterial contamination, serving as an alternative to common antibiotics.
Cell Culture and Transfection
  • TransSerum® EQ Fetal Bovine Serum: Promotes rapid cell growth without mycoplasma contamination and maintains low endotoxin levels.
  • TransIntroTM EL and PL Transfection Reagents: Offer high transfection efficiency with low cytotoxicity, suitable for various cell types and applications.
Cell Detection
  • TransDetect® Luciferase Reporter Assay Kits: Provide rapid and sensitive detection of luciferase activity with minimal interference.
  • TransDetect® Cell Counting Kit (CCK): Offers fast and sensitive cell viability assessment with low cytotoxicity.
  • TransDetect® Apoptosis Detection Kits: Use Annexin V-FITC/EGFP and PI staining to differentiate apoptotic stages.
  • TransDetect® Cell LIVE/DEAD Viability/Cytotoxicity Detection Kit: Differentiates live and dead cells using fluorescence microscopy and flow cytometry.
  • TransDetect® EdU Imaging and Flow Cytometry Kits: Detect cell proliferation through EdU incorporation, offering high sensitivity and specificity without antibodies.
Overview: The document details the TransDetect® EdU Detection Kits, focusing on A549 cell proliferation detection using various fluorophores. The kits use EdU, a nucleoside analog, to detect cell proliferation through DNA replication activity.
Specifications: Designed for easy use without antibodies, the kits offer high sensitivity and specificity, suitable for drug screening, cell proliferation assays, and cytotoxicity assays.
Procedures: The method involves EdU incorporation into newly synthesized DNA during replication, followed by fluorophore labeling through a click reaction. This rapid and sensitive method highlights DNA replication activity in the S phase of the cell cycle.
Comparison with Traditional Methods: The EdU-based method is simpler than the traditional BrdU method, eliminating additional steps like antibody labeling, and provides higher sensitivity and specificity.
Flow Cytometry Results: Includes flow cytometry results for A549 cell proliferation using fluorophores 488, 555, 594, and 647, each providing distinct results for negative and EdU-labeled samples.
Product Range: Lists various TransDetect® kits, including Single-Luciferase Reporter Assay Kits, Double-Luciferase Reporter Assay Kits, Cell Counting Kits, and Apoptosis Detection Kits, available in different specifications.
Applications and Publications: References a publication using the TransDetect® Cell Counting Kit, highlighting its application in genome integrity research.
Contact Information: Provides contact details for TransGen Biotech, including their website, email, customer service number, and phone number.
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Catalog excerpts

Cell biology Related Antibody-1

CELL BIOLOGY Product Series

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Cell biology Related Antibody-2

CONTENTS Mycoplasma Detection TransDetect® Luciferase Mycoplasma Detection Kit ………………………………………………… 1 TransDetect® PCR Mycoplasma Detection Kit ………………………………………………………… 1 TransSafeTM Mycoplasma Elimination Reagent (TransMyco-1+2) …………………………………… 2 TransSafeTM Mycoplasma Elimination Reagent (TransMyco-3) ……………………………………… 2 TransSafeTM Mycoplasma Prevention Reagent ………………………………………………………… 2 Cell Culture and Transfection TransSerum® EQ Fetal Bovine Serum ……………………………………………………………………… 3 TransIntroTM EL Transfection Reagent ……………………………………………………………………… 4 TransIntroTM PL Transfection Reagent ………………………………………………………………………...

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Cell biology Related Antibody-3

Mycoplasma Detection TransDetect® Luciferase Mycoplasma Detection Kit This product is designed using an enzyme unique to mycoplasma. This enzyme degrades MycoDetect Substrate and converts ADP to ATP. Luciferase catalyzes the oxidation of luciferin to produce bioluminescence in the presence of ATP. Cell cultures can be characterized for the presence of mycoplasma contamination by detecting bioluminescence. As this assay can only detect bioactive mycoplasma, the results will be more accurate than that of the PCR assay. The method has high sensitivity, simple operation and time saving. 600000 6×105...

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Cell biology Related Antibody-4

TransSafeTM Mycoplasma Elimination Reagent (TransMyco-1+2) TransSafeTM Mycoplasma Elimination Reagent (TransMyco-3) TransSafeTM Mycoplasma Elimination Reagent (TransMyco-1+2) contains two different and effective anti-mycoplasma components, namely the macrolide antibiotic TransMyco-1 and the tetracycline derivative antibiotic TransMyco-2, which can effectively interfere with the protein expression of mycoplasma. The sequential joint use of these two antibiotic reagents can eliminate mycoplasma contamination effectively, and with the advantages of low concentration and low cytotoxicity. TransSafeTM...

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Cell biology Related Antibody-5

Cell Culture and Transfection TransSerum® EQ Fetal Bovine Serum (Australian healthy fetal bovine blood is used as raw material.) ★ No mycoplasma pollution, extremely low endotoxin levels. ★ The cultured cells grow rapidly, the doubling time is short, and high cloning rate . Successfully cultured cell types with TransSerum® EQ Fetal Bovine Serum 5637 TransSerum® EQ Fetal Bovine Serum on different cell cultures Company TH

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TransIntroTM EL Transfection Reagent ★ High transfection efficiency: Improve the transfection efficiency of a variety of difficult-to-transfect cells. ★ Low cytotoxicity: Minimum effect on cellular physiological processes. ★ Simple operation: No need to change the medium after transfection, tolerance with serum and antibiotics. ★ Wide range of applications: Applicable for both DNA and RNA. Successfully transfected cell types with TransIntroTM EL Transfection Reagent Transfected with DNA (HeLa) Co-transfected with DNA and siRNA (HEK-293) siRNA transfection effect with HEK-293 cells b

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TransIntroTM PL Transfection Reagent ★ Highly efficient and stable transfection for tumor cell lines, such as lung cancer, colorectal cancer, liver cancer, pancreatic cancer, facilitating to establish stable and reliable cell models. ★ Low cytotoxicity and minimum effect on cellular physiological processes. ★ Tolerance with antibiotics. Successfully transfected cell types with TransIntroTM PL Transfection Reagent Tumor cell lines Neural cell lines Other cell lines products series Vero Trans

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Product Name TransSerum® EQ Fetal Bovine Serum TransIntroTM PL Transfection Reagent TransIntroTM EL/PL Transfection Reagent Cell Detection TransDetect® Single-Luciferase Reporter Assay Kit TransDetect® Double-Luciferase Reporter Assay Kit The activity of firefly luciferase reporter is detected by bioluminescent reaction with beetle luciferin as substrate while that of Renilla luciferase is detected by bioluminescent reaction with coelenterate-luciferin. ★ Rapid detection. ★ High sensitivity. ★ Broad range. ★ Free interference from cell endogenous activity. TransGen TransGen Firefly Firefly Renilla...

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TransDetect® Annexin V-FITC/PI Cell Apoptosis Detection Kit TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit Untreated Untreated Camptothecin-treated Camptothecin-treated Annexin V-FITC Annexin V-FITC Propidium Iodide Propidium Iodide Propidium Iodide Propidium Iodide Propidium Iodide Propidium Iodide Camptothecin-treated Camptothecin-treated Untreated Untreated Propidium Iodide Propidium Iodide ★ With AnnexinV-FITC/EGFP as a probe, apoptotic adherent cells and suspension cells can be detected by flow cytometry or fluorescence microscopy. ★ The combination of Annexin V-FITC/EGFP and...

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TransDetect® Cell LIVE/DEAD Viability/Cytotoxicity Detection Kit ★ Higher specificity. ★ More simplicity. ★ Lower cytotoxicity. Green Fluorescence Dead cell Live cell Dead cell Calcein+PI double color stained PI single color stained Live cell Calcein-AM single color stained Green Fluorescence Green Fluorescence The HeLa living cells and the fixed permeabilized dead cells were mixed at a ratio of 4:1, and the results were analyzed by flow cytometry after double staining. Fluorescence microscopy results of double stained Hep G2 cell

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