ugo basile TRANSFORMING IDEAS INTO INSTRUMENTS PAIN and INFLAMMATION www.ugobasile.com TPP Set-Up for Thermal Place Preference Cat. No. 35250 / 35260 General Both heat and cold evoke thermosensation, which, may elicit feelings of pain. Thermosensation is an essential sensory function which involves a variety of transducer molecules. The TPP (or 2-choice temperature test), is a thermal sensitivity assessment tool designed to emphasize integrated learned responses to thermal stimuli applied by the surface on which the animal stands. It documents escape behaviour in awake, unrestrained animals to innocuous and noxious heating or clooling of the floor; rodents learn to minimize pain by escaping to the opposite side; escape latency can be recorded. The Ugo Basile Thermal Place Preference Test fallows monitoring temperature preferences and nociceptive thresholds in rodents (mice and rats), by assessing the rodent's temperature preference. The TPP Test allows an unrestrained animal (either rat and mouse) to move freely between two 2 compartments set at different temperatures, thus choosing its preferred position (comfort zone). for Rats for Mice MONITORING NOCICEPTION THRESHOLDS IN BOTH HOT AND COLD STIMULATION Main Features • OPERATING TEMPERATURE: Hot/Cold Plate: adjustable in the range -5.0 to 65.0°, with 0.1° increments Hot Plate: from room temperature to 65° • PRECISION: +/- 0.1° • CONTROLS: 4"3 touch-screen to set and monitor the test • OPERATING MODES: fixed or ramping temperature, for dynamic experiments • DATA PORTABILITY: via the USB Memory-Key, included as standard

TPP THERMAL PLACE PREFERENCE Instrument Description The Ugo Basile Set-UP for TPP consists of: The TPP Test allows an unrestrained rodent to move freely between two compartments set at different temperatures, via the metal bridge which connects the two areas, thus choosing its preferred position (comfort zone). This behavioural protocol provides data about temperature preferences and nociceptive thresholds associated to both hot and cold stimulation. l an additional Hot Plate 35150-002 l a set including special tubes, and a corridor for either mouse or rat (respectively 35250-003 and 35260-002),...