Cut loose from manual buffer exchange Buffer exchange is the time consuming, hands-on chore you have to deal with before all the things you really want to do. Big Tuna automates the entire tedious process and puts all the control at your fingertips. Exchange one protein, 96 or anything in between. It handles big and small volumes with high precision, so you’ll sail through exchanges without a hitch. 1 – 96 formulations 0.1 – 192 mL sample 30 min hands-on time Up to

Go big or keep it small Big Tuna serves up two consumables to tackle any volume requirement. Use Unfilter 96 to exchange as little as 100 µL on as many as 96 different samples at the same time. Unfilter 24 lets you go big and exchange as much as 8 mL on up to 24 samples. No matter how you roll, Big Tuna can handle it.

Always come out even Big Tuna keeps buffer exchange even across the plate with its unique pressure-based UF/DF and gentle mixing. Its acoustic sensor measures the volume in every well before and after each cycle to track every sample’s exchange rate. Then Big Tuna figures out on the fly how much buffer to add to even things out. Measure volume Measure volume Pressure Input samples Exchanged samples Concentrate (optional)

Be a control freak Buffer exchange doesn’t have to be a shot in the dark or keep you anchored to the bench. Big Tuna gives you full, hands-off control over how efficiently the process happens. Take it slow and easy with delicate proteins or fast-track the sturdy ones. Big Tuna nails the final protein concentration and gets you to next steps quicker.

Know it all You’ll be a Big Tuna expert on the first try. Use the software wizard to drag and drop your proteins and buffers into place — Big Tuna handles the rest. It’ll tell you exactly where to put everything else and how much buffer you’ll need. After the run starts, check in on progress anytime and know exactly when your exchange is

Specifications Application Buffer exchange volume range Unfilter 24: Up to 24 formulations in parallel Unfilter 96: Up to 96 formulations in parallel Exchange time at full volume (96%, at 10 mg/mL IgG) Unfilter 24: 4.5 hours* Unfilter 96: 3 hours* Protein concentration (range) Target concentration accuracy Protein recovery System Volume measurement Ultrasonic sensor Exchange pressure Operating temperature Room temperature Buffer exchange orbital mixing Unfilter 24: Optimized at 700 rpm Unfilter 96: Optimized at 875 rpm Duty cycle programmable 96 cm L x 81.2 cm D x 130 cm H, 220 kg (instrument)...

Unchained Labs 6870 Koll Center Pkwy Pleasanton, CA 94566 Phone: 1.925.587.9800 Toll-free: 1.800.815.6384 Email: [email protected] © 2019 Unchained Labs. All rights reserved. The Unchained Labs logo, Big Tuna and the Big Tuna logo are trademarks and/or registered trademarks of Unchained Labs. All other brands or product names mentioned are trademarks owned by their respective organizations. Rev B