ITICHAINED LABS

Quant and sizing mash up Stunner pulls double-duty so you don’t have to. It’s the only system out there that takes UV/Vis concentration and Dynamic Light Scattering (DLS) size measurements from the same sample at the same time. Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot. Without skipping a beat, you can also measure the colloidal stability of your samples or design your own custom apps. Concentration Sizing Polydispersity B22 kD Homebre

Teeny sample, tons of info Just load 2 µL of sample in a Stunner plate—don’t bother with sample prep or dilution. If all you need is a quick fix on one sample, load it up and you’ll be done in a minute. If you’re dealing with a full plate, get 96 concentration measurements in 12 minutes — add on DLS sizing and have both done in an hour. For even heavier workflows, hook it up to your favorite robot to add more oomph. UV/Vis concentration Hydrodynamic diam

DLS and then some Stunner pulls rank on other systems because it’s way, way more than just DLS. It lets you get a grip on what storage, agitation or a change to your process or formulation does to your protein. See if there’s any aggregation, measure the hydrodynamic size, grab polydispersity when you need to check uniformity, and get the exact concentration while you’re at it. DLS Heterogeneous aggregates present

Take quants higher Load your samples straight and scratch the dilutions. Stunner measures any protein from 0.02 mg/mL to 200 mg/mL. Stunner plates use 2 fixed path lengths for rock-solid precision and a huge dynamic range. It can even take your mAbs all the way up to 250 mg/mL by fitting the shape of their spectra and extrapolating to accurate concentrations. Measured IgG Concentration (mg/mL) Target IgG Concentration (mg/mL)

Double down with B22 and kD On the fly data tells you if your sample’s in good shape, or if aggregation is in the cards. Because the concentration of each sample in the series is measured in real time with DLS, you get the most accurate values without breaking a sweat. Pin down what else is going on with your sample, or just check out its other characteristics. Pick a few wavelengths, a background subtraction method, and create your favorite equation. Stunner’s Homebrew tool is wide open, so you can create apps just the way you like them. Absorbance Absorbance mg/mL mg/mL

U1CHAINEDLABS Unchained Labs 6870 Koll Center Pkwy Pleasanton, CA 94566 Phone: 1.925.587.9800 Toll-free: 1.800.815.6384 Email: [email protected] © 2018 Unchained Labs. All rights reserved. The Unchained Labs logo, Stunner and the Stunner logo are trademarks and/or registered trademarks of Unchained Labs. All other brands or product names mentioned are trademarks owned by their respective organizations.