Application Note · UVP Crosslinker
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Application Note · UVP Crosslinker Ultraviolet-based Applications with the UVP Crosslinker: Surface disinfection of bacterial and viral pathogens Introduction Challenge Small-scale high-intensity, highuniformity ultraviolet light Solution Integrating UVP CL-3000 into ultraviolet applications Ultraviolet light is part of the electromagnetic spectrum that spans from 100-400 nm. The ultraviolet spectrum is broken up into three categories, UVA, UVB and UVC (Table 1). On Earth, UVC is blocked by molecular oxygen in the atmosphere, while UVA and UVB readily travel to the Earth’s surface. Anyone who has experienced a sunburn, can testify to the dangerous power of ultraviolet light. While ultraviolet can be harmful, it has helped improve human health from direct applications to sanitation to our fundamental understanding of how UV affects our biology. UV Common Name *Analytik Jena does not manufacture Vacuum UV products Table 1. Ultraviolet Wavelengths

History of Excellence: Ultraviolet-based Applications with the UVP Crosslinker For over 120 years, ultraviolet light has been used in applications for preventing the growth of microorganisms on surfaces, destruction of microorganisms in the air and sterilization of liquids. For example, since 1877, humans have used ultraviolet light for food preservation/sterilization1; In the 1930s, ultraviolet light started being used in operating rooms to sterilize the air2; and on and off since the early 1900s, ultraviolet light has been used to sterilize drinking water3. The same properties that give...

History of Excellence: Ultraviolet-based Applications with the UVP Crosslinker Figure 2. Uniformity of the Analytik Jena UVP Crosslinker equipped with 254 nm UVC bulbs. A) Profile image of the UVP CL-3000 Crosslinker. B) Diagram depicting position of integrated radiometer readings in mJ/cm2 with dimensions and reported coefficient of variation (CV) calculation for total illumination surface (Total CV) and the center of the illumination surface (Center CV). C) Box-whisker plot summary of radiometer readings. Total CV: Mean=104.9, SD=18.16; Center CV: Mean=117.9, SD=7.1. We recommend users...

History of Excellence: Ultraviolet-based Applications with the UVP Crosslinker Viral and bacterial surface disinfection As an example of viral and bacterial surface disinfection on a glass surface, we used the UVP Crosslinker CL-3000 on Aspergillus Niger (A. Niger) and the virus Adenovirus Type 2 (Ad2,) with the following results: Energy levels 333, 1000, 3000, 9000 mJ/cm2 had detectable levels of A. Niger with a log reduction of >3, respectively, compared to no UV treatment control. 9000 mJ/cm2, in particular, had a log reduction of 3.96. Overall, all energy levels displayed a 0.86 to 3.96...

History of Excellence: Ultraviolet-based Applications with the UVP Crosslinker Part Numbers Part Number Replacement UV tube, 8 watt, 254 nm Replacement UV tube, 8 watt, 302 nm Replacement UV tube, 8 watt, 365 nm References 1. eckers, D. C. Photochemical reactions of natural macromolecules. Photoreactions of proteins. J. Chem. Educ. 50, 164 N (1973). 2. intsis, T., Litopoulou-Tzanetaki, E. & Robinson, R. K. Existing and potential applications of ultraviolet light in the food B industry – a critical review. J. Sci. Food Agric. 80, 637–645 (2000). 3. olfe, R. L. Ultraviolet disinfection of...

History of Excellence: Ultraviolet-based Applications with the UVP Crosslinker 16. eyer, H. U., Edmondson, M., Altenburg, L. & Muller, H. J. Studies on mutations induced by ultraviolet in the polar cap of M Drosophila. in Genetics vol. 35 123–124 (428 EAST PRESTON ST, BALTIMORE, MD 21202, 1950). 17. ovie, W. T. A Preliminary Note on the Coagulation of Proteins by Ultraviolet Light. Science 37, 24–25 (1913). B 18. ovie, W. T. The Temperature Coefficient of the Coagulation Caused by Ultraviolet Light. Science 37, 373–375 (1913). B 19. cLaren, A. & Luse, R. Mechanism of Inactivation of Enzyme...