Product Description VAHTS HiFi Universal Amplification Mix for MGI (SI) is an upgraded version of VAHTS HiFi Amplification Mix, it is designed for the amplification of next-generation sequencing libraries prepared for MGI sequencing. Through directed optimization, VAHTS HiFi DNA Polymerase has excellent compatibility of the template and amplification sensitivity. Combined with an optimized buffer system, VAHTS HiFi Universal Amplification Mix for MGI (SI) can achieve highly uniform library amplification, thus significantly improving the quality of sequencing. It contains special protective agent, which enables long-term storage and maintain stable in activity after repeated freezing and thawing. All the reagents have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation. VAHTS HiFi Universal Amplification Mix 600 pi 4 » 600 pi Storage Store at -30 - -151C and transport at SOt. Applications This product is widely applicable to the amplification of following libraries construction: Conventional DNA libraries(WGS library, Targeted Capture sequencing library, Amplicons sequencing library) and RNA library, etc. Notes For research use only. Not for use in diagnostic procedures. 1. It is recommended to use the purified high-quality library template, refer to the respective Instructions for Use. 2. For special libraries, such as low quality and long fragment libraries, the annealing temperature, extension time and number of amplification cycles can be adjusted according to the instructions in the reaction procedure to obtain the best amplification effect. 3. The extension time should not exceed 1 min/kb. Email: [email protected] Web: www.vazyme.com Loc: Red Maple Hl-tech Industry Park, Nanjing, PRC

Reaction System Components Volume VAHTS HiFi Universal Amplification Mix 25 pi Purified Adapter Ligation products x pi * For non-MGI sequencing platforms, the corresponding amplification primer should be replaced. Reaction Program Components Temperature Time a. The annealing temperature should be adjusted according to the Tm value of the primer, and 60^ is recommended for common MGI sequencing platform library. b. For library of special length, the extension time can be prolonged appropriately. c. Select the appropriate number of cycles according to the following table: Input amounts of template...