ForenSeq™ DNA Signature Prep Kit
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Data Sheet: ForenSeq DNA Signature Prep Kit VEROGEN ForenSeq™ DNA Signature Prep Kit Achieve high resolution and exceptional accuracy, even with complex mixtures, or degraded DNA. Highlights • Eliminate the Need for Multiple STR Kits Interrogate 200 genetic markers using a single, streamlined workflow • Access a Wider Range of Informative SNPs A dense set of forensically relevant SNPs provide valuable information not widely available with current technology • Superior Analysis of Challenging Samples Obtain highly discriminating data from less than 100 pg of DNA, even with complex mixtures, or degraded DNA1 • Multiplexing and Rapid Sample Processing Prepare up to 96 libraries simultaneously using a simple plate-based format, and standard lab equipment V___'_/ Figure 1: ForenSeq DNA Signature Prep Kit Workflow—The ForenSeq DNA Signature Prep Kit is part of a fully integrated, sample-to-answer solution, including library preparation, DNA sequencing platform, and data analysis software specifically designed for forensic genomics. Introduction The ForenSeq DNA Signature Prep Kit is part of the Verogen MiSeq FGx™ Forensic Genomics Solution, a complete, fully validated1 DNA-to-data workflow specifically designed for forensic genomics applications (Figure 1). The ForenSeq DNA Signature Prep Kit includes all the required reagents to prepare sequencing libraries* from forensic DNA samples (Figure 2). With a simple plate-based workflow, prepare up to 96 DNA samples for Illumina next-generation sequencing (NGS), the most trusted and widely adopted technology in the industry.2 Table 1: ForenSeq DNA Signature Prep Kit—Forensic Loci Included Included Amplicon in DNA in DNA Number of Size Range Primer Primer Global Autosomal STRs Biogeographical Ancestry SNPs a. SNP and STR chromosome locations can be found in the ForenSeq DNA Signature Prep Kit Reference Guide. b. Over 200 markers analyzed when running primer set B. A sequencing “library” is a collection of amplified DNA fragments from a single DNA sample. For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures. MVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVaVa' AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AYyAY/AY/AY/AYyAY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AY/AYyAY/AY/AY/A''

Data Sheet: ForenSeq DNA Signature Prep Kit Suspect Yes Suspect Included Suspect Excluded Investigative Leads Figure 3: ForenSeq DNA Signature Prep Kit Forensic Loci and Investigative Workflow—With ~200 genetic markers in a single workflow, the MiSeq FGx Solution offers the most comprehensive multiplex of STRs and SNPs and the most straightforward path to human identification. Eliminate the Need for Multiple STR Kits The ForenSeq DNA Signature Prep Kit consolidates all autosomal short tandem repeat (STR) markers currently used around the world for casework and criminal DNA databasing, into...

Data Sheet: ForenSeq DNA Signature Prep Kit Intact HMWgDNA STR (DYS635) Allele Name: 23 Length (bp): 274 Intensity (# of Reads): 625 Partially Degraded gDNA Length (bp) Figure 4: Analysis of High Molecular Weight vs. Degraded DNA—Comparison of high molecular weight gDNA and partially degraded gDNA performance in ForenSeq DNA Signature Prep Kit. Most ForenSeq SNPs (black circles) are < 125 bp in length, allowing the most information to be extracted from an unknown sample. Powerful population statistics are generated, even when all or most STRs (blue circles) are lost. When no suspect is...

Data Sheet: ForenSeq DNA Signature Prep Kit Sample Types gDNA, buccal swabs, FTA card Table 3: ForenSeq DNA Signature Prep Kit—Kit ContentsReagent DescriptionBox 1 Recommended Input for Human gDNA per Sample Recommended Input for FTA Card Punch per Sample detects minor contributors at < 5% of major simultaneous analysis of ~200 genetic markers Short Amplicon Detection Accurate Low-Level Mixture Detection Locus Multiplexing Capability Learn More For more on the ForenSeq DNA Signature Prep Kit, visit: www.verogen.com/products LNA1 Library Normalization Additives 1 LNS2 Library Normalization...