ForenSeq™
1 / 4Pages

Data Sheet: ForenSeq Universal Analysis Software VEROGEN ForenSeq™ Universal Analysis Software Interrogate the broadest range of forensically relevant loci with a simple user interface and powerful analysis algorithms. a \ Highlights • Complete, Sample-to-Answer Workflow Manage the sequencing workflow from run setup to data analysis, data portability, and report generation • Perform Automated Data Visualization and Reporting with Simple Graphical User Interface Compare samples automatically, generate population statistics, and view data through an intuitive user interface • Generate Investigative Leads Through Analysis Software Advances Estimate visible traits such as hair color and eye color as well as biogeographical ancestry • Discriminate Further with Comprehensive Sequence Variant Analysis Increase resolution with tools for intra-STR and flanking region variant detection V_) Introduction The ForenSeq Universal Analysis Software is part of a fully validated1 sequencing workflow specifically designed for use in forensic genomics applications. The workflow includes the MiSeq FGx™ instrument and ForenSeq DNA Signature Prep Kit and enables simultaneous analysis of approximately 200 forensically relevant single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs)-including marker sets not routinely available with traditional methods (Table 1). From sequencing library* and file management to complete data analysis and reporting, the analysis software provides the full functionality needed to analyze and interpret the widest range of casework and database samples. For remote labs or criminal justice partners that require access to data independent of the MiSeq FGx instrument, the ForenSeq Universal Analysis Software is sold separately on a standalone, dedicated server. Complete, Sample-to-Answer Workflow The Verogen MiSeq FGx Forensic Genomics Solution uses a pipeline of software applications to perform sequencing runs and complete data analysis (Figure 1). ForenSeq Universal Analysis Software guides the sequencing workflow from run-setup and sample data entry to the final stages of data analysis and report generation. MiSeq FGx Control Software (MCS), installed on the MiSeq FGx instrument control computer, captures flow cell images, operates the flow cell stage, and controls reagent delivery and temperature. During the run, Real-Time Analysis (RTA) software performs image analysis, base calling, and Figure 1: ForenSeq Universal Analysis Software Workflow Table 1: Simultaneous Analysis of Forensically Relevant Loci with ForenSeq Universal Analysis Software Global Autosomal STRs 27 Biogeographical Ancestry SNPs 56 Total Number of Locib > 200 a. SNP and STR chromosome locations can be found in the ForenSeq DNA Signature Prep Reference Guide. b. Over 200 markers analyzed when running primer set B. assigns base-by-base quality scores. ForenSeq Universal Analysis Software then initiates the final stages of analysis, including demultiplexing, sequence alignment, allele calling, genotyping, and reporting. Manage Data with Data Portability Features and Sample History The ForenSeq Universal Analysis Software allows creation of data packages for easy management and portability of sample information. Data can be packaged specifically for archive to a separate data storage location, or copied for discovery requests and data sharing between labs. Document and view both system and user actions within the ForenSeq Universal Analysis Software by enabling visibility of the Sample History. A sequencing “library” is a collection of amplified DNA fragments from a single DNA sample. For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures. 'AVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVA' AVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVAVA

Data Sheet: ForenSeq Universal Analysis Software Figure 2: ForenSeq Universal Analysis Project Reports—Reports are organized by project, sample, comparisons, and phenotypes. Project level reports include CODIS, Project Details, and Flanking Region reports. Sample Genotypes provide all allele information for a specific sample. Comparisons and Phenotypic reports provide their relevant information by sample. Perform Automated Data Visualization and Reporting with Simple Graphical User Interface The Verogen ForenSeq Universal Analysis Software provides an easy data analysis workflow and...

Data Sheet: ForenSeq Universal Analysis Software Figure 3: ForenSeq Universal Analysis Software Sample Details Screen—The Sample Details screen displays overall sample results as an intensity plot, along with detailed summary tables for all loci included in the sequencing library. Figure 4: ForenSeq Universal Analysis Software Locus Details Screen—The pop-up Locus Detail screen shows the allele call, intensity bar graph, and stutter. View the Repeat Sequence column to see the full, base-by-base target sequence, and any intra-STR variation present in the sample. Figure 5: ForenSeq Universal...

Table 2: ForenSeq Server Components and Specifications 4 x Seagate 1 TB Sata hard drives RAID controller allowing for data redundancy and speed Intel 2.4 GHz x 64 processor with 6 cores/12 threads Intel socket R3 server board 32 GB DDR4 RAM 550 W power supply ULL, FCC, CE certified Windows Server 2012 R2 Standard with 5 CALs ForenSeq Universal Analysis Software and Server SE-550-1001 Summary ForenSeq Universal Analysis Software contains comprehensive sample management and analytical capabilities, including sample and index management, application-specific workflows, data visualization at...