MiSeq FGx™
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Data Sheet: Verogen MiSeq FGx Forensic Genomics Solution VEROGEN Verogen MiSeq FGx™ Forensic Genomics Solution Solve more cases and generate more leads with the power and accuracy of the Verogen massively parallel sequencing solution. Highlights • Fully Validated, Sample-to-Answer Solution All-inclusive system ties library preparation, sequencing, data analysis, and reporting into a single integrated protocol • Simple, Streamlined Workflow Interrogate 200 genetic markers in a single, streamlined workflow eliminating the need for multiple STR kits • Access a Wider Range of Information Biogeographical ancestry, phenotypic-informative SNPs, and sequence variants provide valuable information not widely available with current technology • Superior Analysis of Challenging Samples Advanced capacity to handle challenging samples such as complex mixtures or degraded DNA V____J Introduction The Verogen MiSeq FGx Forensic Genomics Solution is the first fully validated1 sequencing workflow specifically designed for use in forensic genomics applications (Figure 1). With the high resolution and unmatched accuracy of massively parallel sequencing technology, the MiSeq FGx Solution can transform the most fragile, degraded, or mixed samples into powerful results. A Complete Sample-to-Answer Solution The MiSeq FGx Solution delivers a complete DNA-to-Data workflow for the analysis of forensic DNA samples. The solution begins with the ForenSeq™ DNA Signature Prep Kit, which includes all reagents required to prepare DNA libraries for sequencing (Figure 2). The MiSeq FGx Reagent Kit provides sequencing reagents, an RFID labeled reagent cartridge, and wash solution, which are then loaded onto the Illumina MiSeq FGx instrument along with the sequencing-ready DNA libraries. The intuitive touch screen interface provides simple, step-by-step guidance through each stage of the sequencing run including library and reagent loading, run configuration, and run monitoring. ForenSeq Universal Analysis Software delivers a powerful suite of forensic analysis capabilities including automatic detection of mixed DNA samples, generation of population statistics, and CODIS-compatible reports, as well as an optional off-line report to enable flanking region analysis. The software also enables estimation of visible traits and biogeographical ancestry markers that can provide crucial investigative leads in “no suspect” cases. Built-in automation of the on-board cluster generation and analysis initialization means minimal hands-on time is required. Figure 2: ForenSeq DNA Signature Prep Kit—The ForenSeq DNA Signature Prep Kit includes all reagents required to prepare DNA libraries for sequencing, including PCR reagents, index adapters, and purification and normalization beads. Simple, Streamlined Workflow With current forensic capillary electrophoresis (CE) methods, limited DNA quantities may require forensic analysts to choose between available fragment length-based short tandem repeat (STR) tests. These trade-offs can result in lengthy, complex decision trees as technical limitations are weighed against potentially informative genetic data. The MiSeq FGx solution eliminates the need to choose between relevant STR tests by providing all currently available STR typing tests in a single workflow (Figure 3). One run on the Illumina MiSeq FGx instrument simultaneously interrogates ~200 genetic markers, including a combination of autosomal, X-, and Y-STRs. Furthermore, the ability to obtain highly discriminating data from less than 100 pg of DNA1 enables testing of a broad range of samples where DNA quantity is limited. For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.

Access a Wider Range of Informative SNPs Another casework challenge occurs when an autosomal STR profile is established, but no suspect is available for direct comparison, or no hits are found in national or local criminal databases. In addition to providing a complete set of autosomal STR markers currently utilized around the world for casework and criminal DNA databasing, the ForenSeq DNA Signature Prep Kit also contains marker sets not routinely available with traditional CE methods. These include a dense set of identity informative single nucleotide polymorphisms (iiSNPs),2-3 which are...

Data Sheet: Verogen MiSeq FGx Forensic Genomics Solution Figure 4: ForenSeq Universal Analysis Run Overview Screen—ForenSeq Universal Analysis Software features DNA library management tools, data analysis and visualization, and automated reporting. The Run Overview screen features easy data entry fields for Run Name and Run Description. Sample information, index combinations, and application options can also be imported with a tab delimited .txt file. Sample types include Known Sample, Forensic Sample, Reagent Blank, Negative Amplification Control, Positive Amplification Control, and Human...

Table 2: Illumina MiSeq FGx Instrument Specifications Instrument Configuration RFID tracking for consumables MiSeq FGx Control Software Instrument Control Computer (Internal)a Base Unit: Intel Core i7-2710QE 2.10 GHz CPU Memory: 16 GB RAM Hard Drive: 750 GB Operating System: Windows 7 embedded standard Operating Environment Temperature: 22°C ± 3°C (66-77°F) Humidity: Noncondensing 20%-80% Altitude: Less than 2,000 m (6,500 ft) Air Quality: Pollution degree rating of II Ventilation: Maximum of 1,364 BTU/h For Indoor Use Only Light Emitting Diode (LED) 530 nm, 660 nm Dimensions WxDxH: 68.6 cm...