Cellular immunity
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Cellular immunity Stimulation of cell-mediated immune response by antigens CMV, HSV, VZV IFNγ and IL-2 cytokines detection Virus-specific cell-mediated immunity testing against: • • • • CMV (cytomegalovirus) VZV (varicella zoster virus) HSV-1 (herpes simplex virus 1) HSV-2 (herpes simplex virus 2) Diagnostic relevance: • n patients with high risk of severe I infection - transplanted patients - transplant donors - patients with recurrent infections VIDIA spol. s r.o. Nad Safinou II/365, Vestec 252 42 Jesenice u Prahy Czech Republic • atients treated with immunotherapy P and chemotherapy • Immunodeficient patients • Monitoring of vaccination efficiency

Cellular immunity testing procedure using VIDIA antigens and kits ELISA-VIDITEST Interferon γ, ELISA-VIDITEST IL-2 1. Leukocytes isolation and their stimulation with the specific or control antigen Antigen dilution Cultivation 24 - 48 hod. Leukocytes separation Cells washing and dilution to 2.106 /mL and further dispensing into the plate Cytokines production Cytokines detection using ELISA-VIDITEST Interferon γ ELISA-VIDITEST IL-2 2. Detection of the produced cytokines using ELISA-VIDITEST kits Addition of the detection antibody Addition of standards and samples 2 hours incubation Addition...

*reactivity was defined by IFNy concentration in leukocyte culture medium stimulated by the antigen in vitro (reactive = measurable IFNy concentration) VIDIA spol. s r.o. Nad Safinou II/365, Vestec 252 42 Jesenice u Prahy Czech Republic

• High efficiency of the specific leukocytes stimulation • CMV - BI combi and VZV - BI combi are the complex antigens, which stimulate CD4+ and CD8+ T-lymphocytes • The detection of IFN y and IL-2 allows better characterisation of the status of cell-mediated immunity (detection of the effector and memory T-lymphocytes) • High sensitivity of ELISA-VIDITEST Interferon y and ELISA-VIDITEST IL-2 kits allows the cellular immunity evaluation in the immunocompromised patients VIDIA spol. s r.o. Nad Safinou II/365, Vestec 252 42 Jesenlce u Prahy Czech Republic